2. A B Bait site: Jk5-coding end (CE)
Results based on HTGTS-Rep-seq pipeline A
Vks (~160)
Jks (5)
Cen.
Tel.
Jk5
Non-productive
Productive B
33%
Wild-type v-Abl line
Vk1-88
Vk4-86
67%
32%
E88 v-Abl line
mm9
68%
39%
Wild-type small pre-B
mm9
E88 small pre-B
Vk4-86
Vk1-88
61%
36%
64%

3. A B Bait site: Jk4-coding end (CE)
Results based on HTGTS-Rep-seq pipeline A
Cen.
Tel.
Jks (5)
Vks (~160)
Non-productive
Productive
Jk4 B
36%
Wild-type v-Abl line
mm9
E88 v-Abl line
Vk4-86
Vk1-88
64%
33%
67%
Vk1-88
38%
Wild-type small pre-B
mm9 6 8
E88 small pre-B
Vk4-86
62%
38%
62%

4. A B Bait site: Jk2-coding end (CE)
Results based on HTGTS-Rep-seq pipeline A
Cen.
Tel.
Jks (5)
Vks (~160)
Non-productive
Productive
Jk2 B
30%
Wild-type v-Abl line
mm9
E88 v-Abl line
Vk1-88
Vk4-86
70%
33%
67%
41%
Wild-type small pre-B
mm9
E88 small pre-B
Vk1-88
Vk4-86
59%
39%
61%

5. A B Bait site: Jk1-coding end (CE)
Results based on HTGTS-Rep-seq pipeline A
Cen.
Tel.
Jks (5)
Vks (~160)
Jk1
Non-productive
Productive B
41%
Wild-type v-Abl line
Vk1-88
Vk4-86
59%
33%
E88 v-Abl line
mm9
67%
Wild-type small pre-B
Vk1-88
50%
Vk4-86
50%
41%
E88 small pre-B
mm9
59%

6. Bait site: Jk5-coding end (CE)
Results based on HTGTS translocation pipeline and normalized to the same junction numbers.
Vks
Jks
Igk annotation*
Vk1-88
Vk4-86
(+) 460
Wild-type v-Abl line#
(-) 481
(+) 460
E88 v-Abl line
(-) 481
(+) 988
Wild-type small pre-B$
(-) 1358
(+) 988
E88 small pre-B
(-) 1358
* Red bars denote Vks with RSSs the same directions as the downstream Jk RSSs; blue bars denote Vks with RSSs convergently to the downstream Jk RSSs. Grey bars denote pseudo/ORF Vks.
# (+) means inversional joining between Jk RSSs and the Vks RSSs that are in the same direction as Jk RSSs. (-) means deletional joining between Jk RSSs and the convergent Vks RSSs.
Between these two v-Abl lines with Jk5-CE bait primer, the total junction numbers were normalized to 21,398.
$ Between these small pre-B lines with Jk5-CE bait primer, the total junction numbers were normalized to 50,549.

7. Bait site: Jk4-coding end (CE)
Results based on HTGTS translocation pipeline and normalized to the same junction numbers.
Vks
Jks
Igk annotation
(+) 250
Vk1-88
Vk4-86
Wild-type v-Abl line #
(-) 250
(+) 250
E88 v-Abl line
(-) 258
(+) 233
Wild-type small pre-B $
(-) 377
(+) 233
E88 small pre-B
(-) 377
# Between these two, v-Abl lines with Jk4-CE bait primer, the total junction numbers were normalized to 8,230.
$ Between these small pre-B lines with Jk4-CE bait primer, the total junction numbers were normalized to 7,705.

8. Bait site: Jk2-coding end (CE)
Results based on HTGTS translocation pipeline and normalized to the same junction numbers.
Vks
Jks
Igk annotation
Vk1-88
Vk4-86
(+) 84
Wild-type v-Abl line #
(-) 200
(+) 84
E88 v-Abl line
(-) 200
(+) 245
Wild-type small pre-B $
(-) 338
(+) 245
E88 small pre-B
(-) 338
# Between these two, v-Abl lines with Jk2-CE bait primer, the total junction numbers were normalized to 6,765.
$ Between these small pre-B lines with Jk2-CE bait primer, the total junction numbers were normalized to 9,288.

9. Bait site: Jk1-coding end (CE)
Results based on HTGTS translocation pipeline and normalized to the same junction numbers.
Vks
Jks
Igk annotation
Vk1-88
Vk4-86
(+) 115
Wild-type v-Abl line #
(-) 206
(+) 115
E88 v-Abl line
(-) 206
(+) 153
Wild-type small pre-B $
(-) 152
(+) 153
E88 small pre-B
(-) 152
# Between these two, v-Abl lines with Jk1-CE bait primer, the total junction numbers were normalized to 4,449.
$ Between these small pre-B lines with Jk1-CE bait primer, the total junction numbers were normalized to 4,167.