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Microscopes and Other Tools
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DISSECTING MICROSCOPE
Allows you to see the surface of the specimen Light reflects off the surface of specimen Organisms can be alive Image is in color Seen in 3-D Not for viewing cells
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COMPOUND MICROSCOPE Used to view cells
Magnification up to 1500 X (Most up to 400X) Image appears upside down and backwards We can look at living cells and small organisms Specimen must be cut very thin because light must shine through
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SCANNING ELECTRON MICROSCOPE
Allows you to see surface of specimen Specimen is covered in a thin layer of gold which allows electrons to “bounce off” Viewed in a vacuum (all air is removed) Specimen is NOT alive Seen in black and white and in 3D
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PHASE CONTRAST MICROSCOPE
Widely used for examining such specimens as biological tissues. Enhances contrasts of transparent and colorless objects Able to show parts in a cell or bacteria, which would be very difficult to see in an ordinary light microscope. Enables viewer to see in 3D
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CENTRIFUGE Spins test tubes at a very high rotation speed
Separates out the parts of the liquid by density Ex. Separates blood into RBC, WBC, Plasma and Platelets
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ULTRACENTRIFUGE Separates all of the organelles in a cell
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GEL ELECTROPHORESIS Used to analyze DNA
DNA is cut into pieces and “banding pattern” is seen Pieces of DNA move through an “electric” field
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