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Aggregatibacter actinomycetemcomitans biofilm can be inactivated by methylene blue- mediated photodynamic therapy  Letícia Heineck Alvarenga, Renato Araujo.

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Presentation on theme: "Aggregatibacter actinomycetemcomitans biofilm can be inactivated by methylene blue- mediated photodynamic therapy  Letícia Heineck Alvarenga, Renato Araujo."— Presentation transcript:

1 Aggregatibacter actinomycetemcomitans biofilm can be inactivated by methylene blue- mediated photodynamic therapy  Letícia Heineck Alvarenga, Renato Araujo Prates, Tania Mateus Yoshimura, Ilka Tiemy Kato, Luis Cláudio Suzuki, Martha Simões Ribeiro, Luis Rodolfo Ferreira, Sílvio Antônio dos Santos Pereira, Elizabeth Ferreira Martinez, Eduardo Saba-Chujfi  Photodiagnosis and Photodynamic Therapy  Volume 12, Issue 1, Pages (March 2015) DOI: /j.pdpdt Copyright © 2014 Elsevier B.V. Terms and Conditions

2 Figure 1 The effect of APDI on A. actinomycetemcomitans survival in Log10 cfu/ml. Biofilm was incubated with 100μM MB for 1min, and irradiated (λ=660nm). Data are the means of three independent experiments and the bars are the standard deviation. ***p= Photodiagnosis and Photodynamic Therapy  , DOI: ( /j.pdpdt ) Copyright © 2014 Elsevier B.V. Terms and Conditions

3 Figure 2 Scanning electron microscopy of A. actinomycetemcomitans biofilm is presented in (A) without treatment (control group) and in (B) after 5min APDI. Biofilms were incubated with 100μM MB for 1min and then irradiated for 5min (λ=660nm). The white bars represent 20μm. White arrows indicate bacterial cells and black arrows indicate extracellular matrix. Photodiagnosis and Photodynamic Therapy  , DOI: ( /j.pdpdt ) Copyright © 2014 Elsevier B.V. Terms and Conditions

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