1. English for Chemists
DNA origami
Dresden, 10th December 2013

2. DNA as genetic material
Dresden, 10th December 2013
DNA origami

3. Design new structures with DNA ?!
Dresden, 10th December 2013
DNA origami

4. DNA nanotechnology
DNA nanotechnology or DNA nanoengineering: using DNA to design different structures
only using the structure material of DNA, not the genetic informations
goal: to create a number of more complex (two-dimensional or three-dimensional) nanostructures with a defined structure
Dresden, 10th December 2013
DNA origami

5. Chemical structure of DNA
two polymer chains (double helix) monomers: called nucleotides
backbone: is made a phosphate and a deoxyribose (ester bonds)
four nucleobases: Adenine, Thymine, Guanine, Cytosine
Dresden, 10th December 2013
DNA origami

6. Watson–Crick base pairing
specificity of Watson–Crick base pairing:
only hydrogen interations between:
Adenine Thymine (two hydrogen bonds)
Guanine Cytosine (three hydrogen bonds)
Dresden, 10th December 2013
DNA origami

7. DNA origami
ssDNA (single stranded DNA): plasmid of a bacteriophage, e.g. λ-DNA ‘single-stranded scaffold’ (Einzelstrang-Gerüst)
short oligonucleotides ‘staple strands’
staple and scaffold strands self-assemble in a single step (reason: Watson-Crick base pairing), many of these interactions forms structures
Dresden, 10th December 2013
DNA origami

8. Dresden, 10th December 2013
DNA origami

9. Dresden, 10th December 2013
DNA origami

10. Using DNA origami for …
as a substrate for nanoelectronic circuitry (Schaltkreise)
transport drugs in a DNA origami box …
Dresden, 10th December 2013
DNA origami

11. Thank you for your attention
Thank you for your attention ! I will be happy to answer your questions now.
Dresden, 10th December 2013
DNA origami