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FLOWCYTOMETRY
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What Is Flowcytometry? Flow→ Fluid Cyto→ Cell Metry→ Measurement
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It allows simultaneous multi-parameter analysis of single cells
- Cell Size - Granularity - Cell Surface and Intracellular Molecules - Cell Proliferation & Apoptosis - DNA Analysis
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How Does Flowcytometry Work?
- Fluidics - Optics - Electronics
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Fluidics When the stained cell sample in suspension buffer is run through the cytometer, the tiny “stream” of fluid takes the cells past the laser light one cell at a time.
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Optics Lasers producing light at specific wavelength Scatter
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Forward Scatter = Size of The Particle
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Optics
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Side Scatter = Granularity of The Cell
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For Surface and Intracellular molecules
Antibody Lable
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Fluorescent Compound or Fluorochrome
Labeling of the antibody Fluorescent Compound or Fluorochrome
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Labeling of the antibody
Light Fluorochrome Excitation Emission
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Fluorochromes Surface and Intracellular Staining
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Optics
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Data Analysis
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Good luck
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