1. Date of download: 10/31/2017
Copyright © ASME. All rights reserved.
From: Magnetic Shape Memory Micropump for Submicroliter Intracranial Drug Delivery in Rats
J. Med. Devices. 2016;10(4): doi: /
Figure Legend:
The effect of local TTX application on the gamma power. (a) Lay-out of the experimental setup. The rat (1) was kept at 37 °C on a heating blanket (2) and fixed in a stereotaxic frame (3) by ear bars (4) and incisor bar (5). Electrodes were stereotaxically placed in burr holes (6) and local field potentials were by an MPA8 headstage (7) and AM3600 amplifier (8), digitized by a Power 4101 (9), controlled by spike 2 software (10). Guide cannulas (11) were placed such that the inserted quartz infusion cannula (12) perfused TTX locally. 1 μl TTX solution was supplied through a thin tube (13) driven by the MSM micropump (14) driven by an arduino (15) controlled by pulsed voltage output generated by spike software (16). (b) Local field potentials recorded from hippocampus area CA1 before (top trace), 15 min (middle trace) and 30 min (bottom trace) after the start of a 1 μl TTX application over 3 min. (c) Gamma power was determined as a running average from local field potential recordings in the hippocampus (CA1), visual cortex (VC), and prefrontal cortex (PFC). Gamma power was normalized to the average of the 5 min preceding the TTX application to CA1. Note that TTX reduces gamma power only in CA1.