1. “How does it affect the protein?”
Mutations in DNA
“How does it affect the protein?”

2. ID the mutation as either:
Substitution
Insertion
Deletion
Remember, this is a CHANGE to the original DNA sequence!

3. Identify the type of mutation that has occurred:
Original  ATC TTA GGC CGA
New  ATC TAA GGC CGA
ANSWER : Substitution

4. Identify the type of mutation that has occurred:
Original  ATCTTAGGCCGA
New  ATTAAGGCCGA C
ANSWER : Deletion

5. Identify the type of mutation that has occurred:
Original  ATCTTAGGCCGA
New  ATCTTAGAGCCGA
ANSWER : Insertion

6. Identify the type of mutation that has occurred:
Original  ATCTTAGGCCGA
New  ATCTATAGGCCGA
ANSWER : Insertion

7. There are FIVE results that can occur DUE to a mutation:
Silent mutation  Protein unaffected
Substitution  One amino acid substituted
Premature stop  STOP coded too early and protein is left short
Codon deletion or insertion  ONE amino acid is added in or removed from protein
Frame shift mutation  When a simple insertion or deletion changes the entire sequence of amino acids

8. Identify the type of mutation that has occurred:
Original  ATGTTAGGCCGA
New  ATGTATAGGCCGA
Identify the ORIGINAL protein and NEW protein. What resulted from this insertion?
FRAMESHIFT

9. Identify the type of mutation that has occurred:
Original  ATG TTA GGC CGA
New  ATG TTA GGC CGG
Determine both Original and NEW proteins
Substitution – What results from this?
SILENT MUTATION

10. Identify the type of mutation that has occurred:
Original  ATGTTGATAGGA
New  ATGTTGATTGGA
What results from this Substitution?
Premature STOP