1. From: Enhancement of rAAV2-Mediated Transgene Expression in Retina Cells In Vitro and In Vivo by Coadministration of Low-Dose Chemotherapeutic Drugs
Invest. Ophthalmol. Vis. Sci ;53(6): doi: /iovs
Figure Legend:
Chemotherapeutic drugs increase rAAV2-delivered GFP expression in human retinal pigmented epithelium cells. (A) Representative fluorescence (upper panel) and bright field (lower panel) images of ARPE-19 cells after infection with rAAV2-EGFP alone (a, b), or coadministration of 0.1 μg/mL DXR (c, d), 0.14 μg/mL Ara-C (e, f), 1 μg/mL VP-16 (g, h), or 20 μg/mL DDP (i, j). Imaging was performed 7 days postinfection. (B) FACS analysis of ARPE-19 cells 7 days after infection with rAAV2-EGFP at a viral titer of either 1. 2 , 1. 3 , or vg/cell with or without the presence of chemotherapeutic drugs as indicated. Percentage of GFP-positive cells (B) and mean intensity of GFP fluorescence (C) are plotted. Data are presented as mean ± SD. N ≥ 3. BF, bright field.
Date of download: 11/1/2017
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