1. A B C *** ** Sleep (min/hour) Sleep (min/hour) Fly #60 50 1 50 2 40 40 3
Sleep (min/hour) 30 4 30
Sleep (min/hour)
Fly # 5 20 20 6 7 10 10 8 9 10 12 24
IR beam crossing
Day
Night
Day
(Hours)
Night B
Arousal
Baseline
Startled ** 12 1 10 2 8 3 4
Velocity (pixels/s)
Position in tube 6
Fly # 5 6 4 7 8 2 9 10
Vibration response
1 min
1 min
Baseline
Startled C
General anaesthesia
0.3
Isoflurane gas
1.0
0.2
EC50 (vol.%)
0.8
0.1
0.6
Normalized startle velocity
Startled
0.4
0.2
0.25
0.5
0.75
1.0
Vibration response
in isoflurane
[Isoflurane] volume % ATM