1. CH5. Synaptic transmission
Electrical synpase (bidirection, rapid)

2. Electrical synpase; brainstem (breathing), hypothalamus-hormone release
ATP, second messenger
Cell signaling coupled metabolism in glial cells

3. Synaptic cleft
Synaptic vesicles
Neurotransmitter
Ca2+/ mast cell
Exocytosis

4. Acetylcholine
Co-transmitters
Low frequency activity
High frequency activity

5. Neurotransmitter
pre-synaptic vesicle – glutamate,glycine, asparate
Ca2+ dependent, depolarization dependent release
Specific receptor in postsynaptic neuron

7. Small clear core vesicle-small molecule neurotransmitter
0.5-5 mm/day
Neuropeptide- fast axonal transport (400mm/day)

12. EPP; end plate potential
Curare; inhibitor of Ach receptor
MEPP; miniature end plate potential

13. One vesicle contained 100mM, 10000 molecule- MEPP

15. Local recycling
10-20 sec
1 cycle/min
1 msec

23. Tetrodotoxin-Na channel inhibitor

24. Na+ channel blocker cannot completely block the Action potential
The role of Ca2+ in transmitter secretion

26. Lambert-Easton myasthenic syndrome
(LEMS)
Weakness and fatigability
Presynaptic Ca2+ channel loss
Autoimmune disease- Ca2+ channel Ab
Congenital Myasthenic syndrome
By Ach receptor or Acetylcholine diesterase

27. BAPTA; ca2= chelator

28. Differential release of neurotransmitter

30. Primary target of Ca
Synaptotagmin

31. Botulinum toxin, tetanus toxin protease (SNARE protein cleavage)
A-latrotoxin; induce Ca2+ independent exocytosis (spider venom) ca2+ independent vesicle release
 Neurexin targeting (binding with synaptotagmin)

32. Patch clamp

33. End plate current
End plate potential

36. GABA; Cl- channel
EPSP; excitatory postsynaptic potential
IESP: inhibitory postsynaptic potential

44. KO model
M1-forebrain, seizure-resistant, epilepsy model, IP-related
M2-CNS, heart and Smooth muscle, spinal cord 심장박동, learning and Memory (autoR), 통증,Gi
M3-식욕
M4-autoR, GABA regulation
M5-blood vessel contrcation N1 N2

47. Alpha: 1-10
Beta: 2-4