1. From: Correlation Between Multimodal Microscopy, Tissue Morphology, and Enzymatic Resistance in Riboflavin-UVA Cross-Linked Human Corneas
Invest. Ophthalmol. Vis. Sci ;56(6): doi: /iovs
Figure Legend:
Experimental design and corneal hydration kinetics. (A) Schematic illustration of experimental design: (1) alcohol-assisted epithelial de-epithelialization and corneal suture fixation; (2) RF instillation; (3) confocal and multiphoton imaging; (4) RF-catalyzed UVA irradiation; (5) crosslinking of irradiated corneas in DPBS for 90 minutes and rehydration; (6) multiphoton imaging of cross-linked corneas; (7) immersion of corneas in medium (Alchimia) for restoration of corneal deturgescence; (8) dissection of corneas into two hemispheres; (9) OCT embedment for cryosectioning; (10) MTS histology; (11) collagenase digestion assay of central cornea; (12) data integration and correlation analysis. (B) Time course of corneal hydration kinetics in distilled water, DPBS, medium (Alchimia), and 20% dextran vehicle (n = 4/group). *P < 0.05 compared with medium (Alchimia) at given time points. (C) Representative graphs of corneal turgescent/deturgescent state and corneal thickness after 24 hours immersion in distilled water, DPBS, medium (Alchimia), and 20% dextran vehicle. Scale bar: 2 mm.
Date of download: 11/3/2017
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