1. Protocol of preparing Adhesion Assay imaging
Follow the regular procedure to transfect cell and coat FN;
Each sample pass two dish: one for glass bottom dish (very little); another for cell culture dish;
Before imaging:
Prepare the small dish which coated 1% agarose gel;
Detach the cells of the cell culture dish by 4mM EDTA (without Trypsin) 6-10 min (depend on cell line) in incubator;
Resuspend cells in cell culture medium and plate the cell on the agarose gel dish for 1hr in incubator;
Set up the CO2 chamber; Focus the cell with glass bottom dish on bright field;
Drop all suspending cells onto the glass bottom dish; The cell will be drop-down the glass within 2min;
Imaging:
Search the fluorescent cell and save the position very quickly; From touch glass to spread within 3-5min;