1. Warm-Ups Week #8 Thursday 10/12 Get warm-up sheet out and work quietly
Warm-Ups Week #8 Thursday 10/12 Get warm-up sheet out and work quietly. We’ll Stamp agendas on Monday.
C = Level 0
H = Raise Hand
A = complete your warm-up
M = In assigned seat
P = quietly answering warm-up prompt
S = SUCCESS!

2. Warm-up Prompt – (Level 0) think about it
Warm-up Prompt – (Level 0) think about it. Then, write in your Warm-Ups (2 minutes)
Describe which Adjustment Knobs we use with each objective Lens
AND explain why:
Scanning Objective (4x):
use
Why?
Medium Power Objective (10x):
High Power Objective (40x):

3. Read this to answer the Group Warm-up (2 min.)
Using the microscope
Always observe using the lowest objective first. This allows you to see more of the specimen.
First, focus the image using the COARSE ADJUSTMENT KNOB to raise the stage and bring the object into focus.
Next, bring the object into sharp focus by using the FINE ADJUSTMENT KNOB.
If you need to see more detail, move to a higher power objective.
However, use only the FINE ADJUSTMENT KNOB when using the MEDIUM and HIGH POWER OBJECTIVES. This prevents us from ramming the stage up into the longer objective lenses and possibly damaging them.
Keep both eyes open to reduce eyestrain.
To determine the total magnification of the object, you multiply the power of the eyepiece, or ocular, (10x) by the power of the objective ( x).

4. Now, Talk with Elbow Partners about which Adjustment Knobs you would use with each objective lens and explain why.
(3 Min. at Level 2)
Write the responses that everyone agrees on in your Group Warm-Up Sheet
Scanning Objective (4x):
use
Why?
Medium Power Objective (10x):
High Power Objective (40x):

5. Share group responses with class.

6. Answer to Warm-up Prompt
Describe which Adjustment Knobs we use with each objective Lens
AND explain why:
Scanning Objective (4x):
Use BOTH Coarse and Fine Adjustment Knobs.
Why? To bring the image into rough focus which can then be made sharper, or clearer.
Medium Power Objective (10x):
Use ONLY Fine Adjustment Knob!!
Why? to avoid damaging the longer objective lenses!!
High Power Objective (40x):
Use ONLY Fine Adjustment Knob!!

7. why do scientists use Microscopes?
To see very tiny objects you can’t see with naked eye.
To learn about cells.
To learn about plants and animals.
To diagnose illnesses.
To solve crimes.
To create nanobots and other nanotechnology for Medical Field and Military.

8. Essential Question: How do we use the microscope to learn about very tiny objects?

9. To learn about plants. This is Senecio Vulgaris a. k. a
To learn about plants. This is Senecio Vulgaris a.k.a. “Old-man-in-the-Spring”. It is the Common Groundsel, a flowering plant in the daisy family.

10. We can also learn about Animals.

11. We can learn about microorganisms.

12. The Letter “e” lab: Learning to use the Microscope
Miguel Casillas
6th Grade Integrated Science

13. Purpose of today’s Hands-On Activity is to practice using a microscope.
We will:
Develop skills in using the microscope.
Learn to prepare wet mount.
Observe a specimen and draw it.

14. Materials Compound Light Microscope
Tub and Zip lock containing the following:
3 Glass Slides
3 Cover Slips
Newspaper
2 Scissors
2 Forceps (tweezers)
Ruler
Paper towel
Water
2 Disposable pipettes

15. CHAMPS EXPECTATIONS Small group work with elbow partners.
Conversation
Level 2
reach out – only people you can touch can hear you.
Help
Ask elbow partners.
Activity
Work with elbow partners to complete microscope activity.
Movement
At your own table.
Participation
Speak and listen to your partners to complete activity.
Stay on task and on topic.
Success!
You are now an expert in the use of a microscope.

16. Preparing a Wet Mount Slide
Using a disposable pipet or dropper, add ONE drop of water to a clean microscope slide. (Don’t use too much or the water will run off the edge and make a mess!)
Using forceps, put the specimen (letter “e”) in the water.
Place the edge of a coverslip on the slide so that it touches the edge of the water.
Slowly lower the coverslip to prevent trapping of air bubbles (like closing a book).
Cover Slip
Lower slowly

17. Carrying a Microscope A B

18. When you have made your wet mount slide of the letter “e”,
Bring a microscope to your desk following proper carrying technique.

19. Ocular lens (Eyepiece)
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Always carry a microscope with one hand holding the arm and one hand under the base.
Base

20. Activity 1 Prepare wet mount of letter “e”
Look at specimen under low power and draw what you see.
Repeat with medium power and high power.
Use proper clean-up technique

21. Let’s give it a try ... Turn on the microscope by turning knob.
Use two fingers to rotate the nosepiece to click the scanning objective lens (red-banded) into place.
Place your slide on the stage and secure it using the stage clips.
Adjust the slide so that the letter “e” is in the center of your field of view.
Use the coarse adjustment knob (large knob) to get the image into view and roughly focused. Then, use the fine adjustment knob (small knob) to make it clearer, sharper image.
Draw exactly what you see on your worksheet in Circle a).

22. Use Medium Power Objective Lens
Use two fingers to rotate the nosepiece to click the Medium Power objective lens (yellow-banded) into place.
Be careful with this longer objective lens!
Use only the fine adjustment knob (small knob) to make it a clearer, sharper image.
Draw exactly what you see on your worksheet in Circle b).

23. Use High Power Objective Lens
Use two fingers to rotate the nosepiece to click the High Power Objective lens (Blue-banded) into place.
Be careful with the longest objective!
Use only the fine adjustment knob (small knob) to make it a clearer, sharper image.
Draw in Circle c).
When you are done, turn off the microscope and clean the slides you used. Return slides to the little tub and other supplies to baggie.
**Be careful because glass is sharp!**

24. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images is being viewed at a higher power of magnification?
Explain how you know this.

25. Fill in the table on your worksheet.
What’s my power?
To calculate the Total power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?
Fill in the table on your worksheet.

26. Storing The Microscope
Return the lowest power objective in place.
Return stage to lowest setting.
Wrap the cord and return to teacher or charge microscope.
Replace dust cover.

27. Resources