1. Mitochondrial Ferritin Protects Hydrogen Peroxide-Induced Neuronal Cell Damage
Guofen Gao 1 ;Nan Zhang 1 ;Yue-Qi Wang 1 ;Qiong Wu 1 ;Peng Yu 1 ;Zhen-Hua Shi 1 ;Xiang-Lin Duan 1 ;Bao-Lu Zhao 1 ;Wen-Shuang Wu 2 ;Chang Yan-Zhong 1 ;
1 Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, , China ; 2 The 3rd Hospital of Hebei Medical University, Shijiazhuang, Hebei, , China ;
Figure 1. Effects of FtMt on cell viability and apoptosis ratios of cells treated with H 2 O 2 A The expressions of exogenous mouse FtMt top panel and endogenous human FtMt bottom panel in FtMt overexpressed transfectants FtMt were examined by western blot using anti-mouse and anti-human FtMt antibodies, respectively. K562-Mt6 is a human FtMt overexpressing cell line a gift from Prof. Sonia Levi, which is used as a positive control. B The wild-type WT SH-SY5Y cells, empty vector transfectants vector, and FtMt overexpressed transfectants FtMt were treated with increasing concentrations 0, 20, 40, 60, 80, 100, 120, 140 #cod#x003BC;M of H 2 O 2 for 24 h. Cell viability was measured with the MTT assay. Data were presented as percentage of the cell viability compared with untreated WT control cells #cod#x000B1; SD, n=6 ##, p #cod#x0003C; 0.01 vs. the H 2 O 2 -treated vector control cells. C The relative apoptotic ratios of control and FtMt-SY5Y cells with or without H 2 O 2 treatment 100 #cod#x003BC;M, 24 h were determined by flow-cytometry Ci and Cii. Data were presented as means #cod#x000B1; SD; n=3 #cod#x0002A;#cod#x0002A;, p #cod#x0003C; 0.01 vs. the untreated cells of same genotype; ##, p #cod#x0003C; 0.01 vs. the H 2 O 2 -treated vector controls.
Aging and Disease,null,8(4), Doi: /AD