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From: qPrimerDepot: a primer database for quantitative real time PCR

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Presentation on theme: "From: qPrimerDepot: a primer database for quantitative real time PCR"— Presentation transcript:

1 From: qPrimerDepot: a primer database for quantitative real time PCR
Figure 1 Web input interface for qPrimerDepot (see text). From: qPrimerDepot: a primer database for quantitative real time PCR Nucleic Acids Res. 2006;35(suppl_1):D805-D809. doi: /nar/gkl767 Nucleic Acids Res | Published by Oxford University Press 2006This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

2 From: qPrimerDepot: a primer database for quantitative real time PCR
Figure 2 Web output interface for qPrimerDepot (see text). From: qPrimerDepot: a primer database for quantitative real time PCR Nucleic Acids Res. 2006;35(suppl_1):D805-D809. doi: /nar/gkl767 Nucleic Acids Res | Published by Oxford University Press 2006This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

3 From: qPrimerDepot: a primer database for quantitative real time PCR
Figure 3 Validation of pPrimerDepot primer sets on 3% ethidium bromide stained agarose/acrylamide (3:1) gels after 38 cycles of amplification. Asterisk indicates 125 bp size marker. From: qPrimerDepot: a primer database for quantitative real time PCR Nucleic Acids Res. 2006;35(suppl_1):D805-D809. doi: /nar/gkl767 Nucleic Acids Res | Published by Oxford University Press 2006This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

4 From: qPrimerDepot: a primer database for quantitative real time PCR
Figure 4 Real-time PCR amplification profiles generated using qPrimerDepot primer sets for non intron-bearing (XCR1, SSTR4, MC1R) and intron-bearing (VEFG, VEGFB, VEGFC) genes compared after the addition of increasing amounts of contaminating genomic DNA. From: qPrimerDepot: a primer database for quantitative real time PCR Nucleic Acids Res. 2006;35(suppl_1):D805-D809. doi: /nar/gkl767 Nucleic Acids Res | Published by Oxford University Press 2006This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


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