1. Warm up
Modern technology could be used to clone animals (like you favorite dog). Cloned animals would resemble the original pets because:
How is the process of gene therapy used to treat cystic fibrosis?
Describe the procedure represented in the diagram below using these terms?
Restriction enzyme
Recombinant DNA
Plasmid
Vector

2. Who committed the crime and why?

3. What is similar about these two processes?

4. How could life have begun on a lifeless planet?
Origins of life?
Origins of Life
How could life have begun on a lifeless planet?

5. Abiogenesis/Spontaneous Generation
Abiogenesis is the idea that life came from non-living material.
This idea is sometimes called spontaneous generation.

6. Fill It In …
Abiogenesis =

7. Early Environment
The environment of the early Earth may have provided a unique set of conditions that allowed abiogenesis to occur.

8. Early Environment
Researchers now believe that the early atmosphere may have been similar to the vapors given off by modern volcanoes: carbon monoxide, carbon dioxide, hydrogen sulfide, and nitrogen (note the absence of free atmospheric oxygen).

9. Fill It In …
Gases then =
Gases now =

10. Early Environment
Oparin developed a theory to explain the development of life on earth.
His theory hypothesized that due to the chemicals in the atmosphere, the lack of free oxygen, and intense energy from lightning and volcanoes, simple organic molecules could form from inorganic compounds.

11. Early Environment
At this time in the earth’s history, the earth was covered by water
Therefore, this essential first step in the development of life must have occurred in the oceans.
This supports the idea that life originated as a “primordial soup” in the oceans.

12. Early Environment
Miller and Urey designed an experiment to test Oparin’s “primordial soup” hypothesis
They were able to successfully mimic the proposed conditions of early earth in the laboratory.
Up to 4% of the carbon was converted to amino acids (the building blocks of proteins).
This experiment has been replicated numerous times.

14. Fill It In …
What was found in the “primordial soup”?

15. Biogenesis
Once life was established in very simple cells, biogenesis began.
Biogenesis is the continuation of life from other living cells.
For a long time people believed that nonliving material could produce living things (spontaneous generation).
For example, it was a commom belief that fish arose from the mud in the bottom of a river.

16. Fill It In …
Abiogenesis
Biogenesis

17. Biogenesis
Francesco Redi set out to disprove the theory of spontaneous generation/abiogenesis.
He developed a controlled experiment to test his hypothesis that life must come from life (biogenesis)

18. Redi’s Experiment Control Grp Experimental Grp Independent variable
Open jars
Covered jars
Constant
Rotting meat
Observations
Flies entered jars, landing on the meat
Flies were unable to enter the jar
Results
Maggots developed on meat
No maggots developed on meat
Conclusions
The maggots came from the flies, NOT the meat

19. Biogenesis
After the discovery of the microscope and thus the discovery of microorganisms, Redi’s work was called into question.
Did the microscopic organisms come from a vital force in the air or did biogenesis hold true at all levels?

20. Biogenesis
Louis Pasteur designed an experiment to disprove spontaneous generation for microorganisms

21. Pasteur’s Experiment Experimental group Control group
Conclusion: Microorganisms come from other microorganisms carried on dust in the air, NOT the air itself.

22. Heredity- DNA OR RNA First???
Some experiments show that RNA could have replicated on its own
More simple than DNA
Easier to make (less energy required)

23. The evolution of cells
Based on the conditions proven by Miller and Urey, scientists developed the heterotroph hypothesis to explain the evolution of prokaryotic cells.

24. The evolution of cells
The first cells would have been prokaryotic (no nucleus), anaerobic (does not require oxygen), and heterotrophic (must take in nutrients).
Prokaryotic, heterotrophic cells are the simplest cells and therefore most likely to evolve first
The lack of free atmospheric oxygen would have required an anaerobic cell.

25. The evolution of cells
Over time, photosynthetic prokaryotic cells evolved, allowing for the release of free oxygen.
This profoundly changed earth’s environment and led to the development of an ozone layer.

26. The evolution of cells
The production of oxygen led to conditions that favored the evolution of aerobic, prokaryotic cells.

27. First Cells Which came first Autotrophs or Heterotrophs?
Obligate anaerobes are microorganisms that live and grow in the absence of molecular oxygen; some of these are killed by oxygen
Chemoautotrophs
Photoautotrops
Oxygen in the atmosphere produces mass extinction
Aerobic organism survive
Cellular respiration evolves
Eukaryotic organisms
Multicellular organism

28. Fill It In …
The effect of photosynthetic cells: 1. 2. 3.

29. The evolution of cells
Based on the idea of biogenesis and current research in symbiosis, Lynn Marguilis developed the endosymbiont hypothesis to explain the development of eukaryotic cells.

30. The evolution of cells
A variety of prokaryotic cells existed, some autotrophs and some heterotrophs
A larger heterotrophic cell consumed the smaller cells, using some of them for food. However, the energy harnessing power of these smaller cells could also be used by the larger cells.

31. The evolution of cells Endosymbiotic Theory
A symbiotic relationship was formed and the smaller cells evolved into what we now know as mitochondria and chloroplasts.
Mitochondria and chloroplasts have their own DNA (one circular chromosome … sound familiar?) and a double membrane around the organelle
A nuclear envelope formed around the DNA.

32. First Eukaryotic Cells

33. Eukaryotic Cells
Mitochondria and Chloroplasts contain their own bacterial like DNA and plasma membrane
Have ribosome whose size and structure resemble bacterial DNA
The both reproduce by binary fission when their cells divide by mitosis
Free living bacteria entered cells= eukaryotic cells

34. Check Yourself! What is abiogenesis?
What were the conditions of the early atmosphere?
What three scientists are credited with developing and supporting the theory of these conditions on early Earth?
What is biogenesis?
What two scientists disproved spontaneous generation using controlled experiments?
What does the heterotroph hypothesis explain?
What does the endosymbiont hypothesis explain?

35. Check Yourself! What is abiogenesis?
THE IDEA THAT LIFE CAME FROM NON-LIVING MATERIAL

36. Check Yourself! What were the conditions of the early atmosphere?
NO FREE OXYGEN
INTENSE LIGHTNING
VOLCANOES

37. Check Yourself!
What three scientists are credited with developing and supporting the theory of these conditions on early Earth?
OPARIN
MILLER
UREY

38. Check Yourself! What is biogenesis?
THE CONTINUATION OF LIFE FROM OTHER LIVING CELLS

39. Check Yourself!
What two scientists disproved spontaneous generation using controlled experiments?
FRANCESCO REDI
LOUIS PASTEUR

40. Check Yourself! What does the heterotroph hypothesis explain?
THE EVOLUTION OF PROKARYOTIC CELLS

41. Check Yourself! What does the endosymbiont hypothesis explain?
THE EVOLUTION OF EUKARYOTIC CELLS