1. Standardization of Sample Preparation for NAT
SoGAT XVII
May 27, 2004
Dr. Frank Krieg-Schneider
Strategic Alliances
QIAGEN

2. Standardized systems for sample preparation
Agenda
QIAamp DSP Virus kit
BioRobot M48 for medium throghput

3. Manual sample preparation: QIAamp DSP Virus kit
Features
500µl sample input volume
Serum or plasma
Viral DNA or RNA purified from virus positive samples
Elution in 20 µl or 60 µl
Amplification using commercial or homebrew assays
Probit analysis to determine 95% cut off

4. DSP Virus kit: Linear Range

5. Automated sample preparation on BioRobot M48
6–48 samples per run
Serum or plasma
Citrate- or EDTA-preserved
400 µl sample input volume
Magnetic silica particles

6. MagAttract Virus M48 ― R&D data
Efficient purification: vDNA & vRNA
Typical RNA Virus
Typical DNA Virus

7. MagAttract Virus M48 ― R&D data
Cross-contamination
Alternating +/- samples on worktable
107 c/ml armored RNA
No false-positives detected
Reproducibility

8. Standardizing sample preparation in 2004
QIAamp DSP Virus kit: CE certified in April 2004
BioRobot M48 for medium throghput in May 2004
BioRobot MDx: CE certification in Q3/04
BioRobot EZ1: for low throughput in Q3/04