1. Replacement of PVP by Hyaluronan in ICSI
PVP is a fully undegradable organic compound which may have hazardous effect (Barak et al 2001)
Hyaluronan (Hyaluronic acid) is a natural compound surrounding the oocyte at the time of fertilisation. It binds only to mature spermatozoa (Kovacs et al.2004) and so, allows to select the mature ones.
A comparative study has been performed in 2003
Transfer on D3 after culture in ISM1 medium

2. Replacement of PVP by Hyaluronan in ICSI
Hyaluronan PVP
Patients
Age (Yrs)
Transfer rate 96% 94%
(88/92) (105/110)
No Embryos/tr
(166/88) (195/105)

3. Replacement of PVP by Hyaluronan in ICSI
Hyaluronan PVP
Clin Pregnancies
Implantation rate
Clinical % %
47/ /195
Ongoing % %
42/ /195

4. Replacement of PVP by Hyaluronan in ICSI
Hyaluronan offers a good opportunity to get rid of PVP
It allows selection of the best spermatozoa, giving at least as good results as PVP