1. Validated Liquid Culture Monitoring System for Lifespan Extension of Caenorhabditis elegans through Genetic and Dietary Manipulations
Myat Thu Thu Win 1 ;Yasuhiko Yamamoto 1 ;Seiichi Munesue 1 ;Dong Han 1 ;Shin-ichi Harada 2 ;Hiroshi Yamamoto 1 ;
1 Department of Biochemistry and Molecular Vascular Biology, Kanazawa University Graduate School of Medical Sciences, Kanazawa , Japan ; 2 Center for Biomedical Research and Education, Kanazawa University Graduate School of Medical Sciences, Kanazawa , Japan ;
Figure age-1 hx546 C. elegans survival in the liquid culture system . Kaplan-Meier survival curves and median, mean, and maximal lifespan of age-1 hx546 C. elegans fed alive E. coli or cfuml and UV-killed E. coli or cfuml. Dashed red and blue lines indicate Kaplan-Meier survival curves of WT C. elegans fed cfuml alive E. coli and cfuml UV-killed E. coli , respectively. n = cfuml alive E. coli , n = cfuml UV-killed E. coli , n = cfuml alive E. coli , n = cfuml UV-killed E. coli , Log-rank test, p between alive E. coli at and cfuml, between UV-killed E. coli at and cfuml, between cfuml alive E. coli and cfuml UV-killed E. coli , and between cfuml alive E. coli and cfuml UV-killed E. coli . Values represent the mean ± SE of the sum of 3 independent experiments.
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