1. Kiril Lisichkov , Stefan Kuvendziev E-mail : lkiril@tmf.ukim.edu.mk
SUPERCRITICAL FLUID CO2 EXTRACTION OF BIOACTIVE ANTIOXIDANTS FROM NATURAL RAW MATERIALS
Kiril Lisichkov , Stefan Kuvendziev
Faculty of Technology and Metallurgy University St. Cyril and Methodius Skopje, R. Macedonia

2. THE MAJOR GREEN TECHNOLOGIES
GREEN SOLVENT SEPARATION PROCESSES

3. SUPERCRITICAL FLUID EXTRACTION

5. • • Phase diagram for pure CO2 Pressure (bar) Temperature (ºC) 74
SOLID
SUPERCRITICAL
Critical
FLUID •
point
Pressure (bar) 74
LIQUID
Triple •
point
5.1
GAS 1
-78 ºC
-56.3ºC
31.1ºC
Temperature (ºC)

6. CARBON DIOXIDE ADVANTAGES SC-CO2 AS A GREEN SOLVENT
Similar advantages to water
-Natural, cheap, plentiful (too much of it!)
-Available in >99.9% pure form, £70/$110 per 25kg.
-By-product of brewing, ammonia synthesis, combustion
Already being adopted in a variety of commercial processes
Non-toxic and properties well understood
-asphyxiant at high concentrations
Easily removed and recycled, and can be disposed of with no net increase in global CO2
-Simple product isolation by evaporation, to 100% dryness.
No solvent effluent
Potential for product processing (extraction, particle formation, chromatography etc.)

7. Biological properties of diferrent tomato type
SEPARATION OF NATURAL BIOACTIVE ANTIOXIDANTS FROM PLANT MATERIALS
Biological properties of diferrent tomato type
Natural antioxidants from tomato

8. SFE - CO2 TOMATO PROCESSING TOMATO WASTE (SKINS AND SEEDS)
Hydro separation
TOMATO SKINS (66%)
SFE - CO2

9. INFLUENCE OF THE WORKING PARAMETERS (T, P)
Тemperature ( 0C )
Yield of lycopene (%)
R (mg lycopene/g CO2 ) 40
10,2
0,0032 50
13,3
0,0041 60
14,9
0,0046 65 20
0,0062
Pressure (bar)
Yield of lycopene (%)
R (mg lycopene/g CO2)
200
14,88
0,0046
250
16,83
0,0052
300 20
0,0062

10. As natural raw material for isolation of biological active components different fish samples (raw caviar from fish type Carasius sp.), is applied, and as working fluid for extraction the supercritical CO2 was used.
FATTY ACID COMPONENTS
RAW CAVIAR
(wt %)
C 18:0
0.9 ± 0.2
C 18:1
19.1 ± 0.1
C 18:2
2.4 ± 0.1
C 18:3
0.64 ± 0.4
C 18:4
8.21 ± 0.2
C 20:1
0.17 ± 0.1
C 20:2
2.1 ± 0.3
C 20:3
2.3 ± 0.1
C 20:4 n-6
2.8 ± 0.2
C 20:5 n-3
C 22:2
2.8 ± 0.1
C 22:6 n-3
3.6 ± 0.1
Saturated
12 ± 0.3
MUFA
43.1 ± 0.2
PUFA
40.1 ± 0.1
n-3 FA
4.5 ± 0.1
Trace
another

11. FINAL FISH PRODUCTS
SFE - CO2
FISH OIL

12. e-mail: klisickov@yahoo.com
Thank You for the Attention!