1. by Yong Du, Aqing Yao, Dengfu Guo, Tadashi Inagami, and Donna H. Wang
Differential Regulation of Angiotensin II Receptor Subtypes in Rat Kidney by Low Dietary Sodium
by Yong Du, Aqing Yao, Dengfu Guo, Tadashi Inagami, and Donna H. Wang
Hypertension
Volume 25(4):
April 1, 1995
Copyright © American Heart Association, Inc. All rights reserved.

2. Schematic diagram of the cDNA probes for the angiotensin II type 1 receptor, and its subtypes AT1A and AT1B, derived from the rat AT1A and AT1B genes. +1 indicates transcription initiation sites.
Schematic diagram of the cDNA probes for the angiotensin II type 1 receptor, and its subtypes AT1A and AT1B, derived from the rat AT1A and AT1B genes. +1 indicates transcription initiation sites. Kpn I, EcoRI, and HindIII are the restriction sites. Arrows indicate primer binding sites.
Yong Du et al. Hypertension. 1995;25:
Copyright © American Heart Association, Inc. All rights reserved.

3. Northern blots done with [32P]deoxycytidine triphosphate–labeled angiotensin II type 1 receptor (AT1) (A, upper panel) and AT1 subtype AT1A (B, upper panel) cDNA to hybridize RNA isolated from kidneys of 5 rats consuming a normal-sodium diet (lanes 1, 3, 5, 7, and 9) and 5 rats consuming a low-sodium diet (lanes 2, 4, 6, 8, and 10).
Northern blots done with [32P]deoxycytidine triphosphate–labeled angiotensin II type 1 receptor (AT1) (A, upper panel) and AT1 subtype AT1A (B, upper panel) cDNA to hybridize RNA isolated from kidneys of 5 rats consuming a normal-sodium diet (lanes 1, 3, 5, 7, and 9) and 5 rats consuming a low-sodium diet (lanes 2, 4, 6, 8, and 10). Each lane represents 1 rat (30 μg of total renal RNA). The blots were stripped and rehybridized with [32P]deoxycytidine triphosphate–labeled 18S rRNA cDNA (A and B, lower panels). C and D, Densitometric analyses of Northern blots in A and B, corrected for 18S rRNA in each blot. Results represent mean±SEM. NS indicates rats consuming 0.5% sodium; LS, rats consuming 0.07% sodium. *P<.05.
Yong Du et al. Hypertension. 1995;25:
Copyright © American Heart Association, Inc. All rights reserved.

4. Representative photomicrographs showing the localization of renal AT1B mRNAs in the kidneys of rats fed a normal-sodium diet (A, cortex; B, medulla) and a low-sodium diet (C, cortex; D, medulla) by in situ hybridization.
Representative photomicrographs showing the localization of renal AT1B mRNAs in the kidneys of rats fed a normal-sodium diet (A, cortex; B, medulla) and a low-sodium diet (C, cortex; D, medulla) by in situ hybridization. Photomicrographs showed that tubular cells in the cortex and medulla of the control rats hybridized to digoxigenin-labeled antisense angiotensin II type 1 receptor subtype AT1B cRNA probe (A and B, arrows). Renal AT1B mRNA expression in both the cortex and medulla was markedly decreased by the low-sodium diet (C and D). The black bars represent 100 μm. Photomicrographs hybridized to labeled sense probes (E, cortex; F, medulla) or that were without probes (G, cortex; H, medulla) were devoid of staining in control rats.
Yong Du et al. Hypertension. 1995;25:
Copyright © American Heart Association, Inc. All rights reserved.

5. Quantitative image analysis of in situ hybridization of renal angiotensin II type 1 receptor subtype AT1B mRNA levels in renal cortex (left) and medulla (right) of the rats consuming normal-sodium and low-sodium diets.
Quantitative image analysis of in situ hybridization of renal angiotensin II type 1 receptor subtype AT1B mRNA levels in renal cortex (left) and medulla (right) of the rats consuming normal-sodium and low-sodium diets. Results represent mean±SEM (n=9) in each group. NS indicates rats consuming 0.5% sodium; LS, rats consuming 0.07% sodium. *P<.05.
Yong Du et al. Hypertension. 1995;25:
Copyright © American Heart Association, Inc. All rights reserved.