1. Topics to be covered Principles of immunological detection methods
Formats of ELISA assays, direct, sandwich
Immunoblotting
Substrates for detection
Chromogenic, fluorogenic substrate, fluorescence labeled detection molecules
Other methods, RIA and immunofluorescence microscopy

2. Sandwich ELISA and other formats

3. SDS-PAGE & ImmunoblottingX
Substrate reaction produces a chemiluminescent signal
HRP: horse raddish peroxidase

4. Substrates for detection
Substrate reaction produces a chemiluminescent signal
HRP: horse raddish peroxidase

5. SDS-PAGE & Immunoblotting
Cell extracts and affinity purified samples will be analyzed using SDS-PAGE and immunoblotting
A putative result:
Reducing conditions
130 kDa
α -IgG-HRP
100 kDa
70 kDa
Secretion signal -hc
55 kDa hc
35 kDa
25 kDa
EndoH
IgG 10 ng
HyHEL
-Fv-Fc-fusion
Her2 IgG
HyHEL
IgG

6. Other immunological detection methods
Immunofluorescence
RIA
See also: