1. Classical laboratory filtration setup
Plant cell suspension
Agrobacteria suspension
Classical laboratory filtration setup
Collects plant cells
Vacuum tubing
Vacuum tubing
Rubber ring
valve
pump
We need different rubber rings. One type fitting the buchner funner and another type fitting e.g. the wide side of a blue tip. We can use parafilm for minor adjustments / fittings.
optional: manometer
Collects excess Agrobacteria suspension
„fixing“ gear
1 Liter flasks work well, smaller flasks need to be „fixed“ to ensure that they are not topped over during handling

2. Examples of suitable equipment
Membrane pumps
Rubber rings
plunger pump
Accessoirs:
tube connector
adapter
tweezer
round filter paper …
Filtration Unit
A manual pump, cheap and interesting but i am not sure how good this works…
Buchner Funnel
(plastic / ceramic)

3. Vacuum manifold for columns / multiwell plates