1. 500 mg total protein /mL S-CM 250 mg total protein /mL S-CM
Supplemental Table I. Levels of key immune-modulating cytokines in working concentrations of stroma-conditioned media derived from normal mucosa and inflamed Crohn’s mucosa
Cytokine
(pg/mL)
500 mg total protein /mL S-CM
250 mg total protein /mL S-CM
50 mg total protein /mL S-CM
TGF-b
6 + 2
IL-6 nd
IL-1b
38 + 5
20 + 8
10 + 4
Normal S-CM
Crohn’s S-CM
nd = not detectible

2. Supplemental Figure 1 A B C D T-bet b-actin PHA- stimulated Figure S.150
250
500
S-CM (mg/mL) * **
% Inhibition of Proliferation
Normal S-CM
Crohn’s S-CM
+ Normal
S-CM
50 mg/mL
250 mg/mL
500 mg/mL 10 2 3 4 5
40.5
4.0
1.4
62.9
22.9
35.8
51.3
+ Crohn’s
PHA-
stimulated
IFN-g (pg/mL)
Normal S-CM
(mg/mL)
Crohn’s S-CM 50
250
500 C
T-bet
Media D
b-actin
Normal
S-CM
Crohn’s
Figure S.1

3. % Inhibition of Proliferation % Inhibition of IFN-g Release
Supplemental Figure 2
Normal S-CM
Crohn’s S-CM
Proliferation
IL-2
CD3
PHA
% Inhibition of Proliferation
p =0.021
p =0.052
p =0.035 B A
19.7
3.3
7.3
IL-2 64 39
59.8
IL-2 + CD3
61.6
17.9
39.1
IL-2 + PHA
Media
(D. 3-7)
Normal S-CM
(D. 3-7) C
p =0.25
p =0.0005
p =0.006
IL-2
CD3
PHA
% Inhibition of IFN-g Release
Normal S-CM
Crohn’s S-CM
IFN-g
Crohn’s S-CM
(D. 3-7)
Days 0-3: IL-2 + CD3/CD28 or PHA
Days 3-7: CFSE + Crohn’s or Normal S-CM
Day 7: Harvest cells and measure proliferation and cytokines in supernatant

4. % Inhibition of Proliferation % Inhibition of IFN-g Release
Supplemental Figure 3
IL-2
CD3
PHA
% Inhibition of Proliferation
p =0.009
p =0.011
p =0.014
Normal S-CM
Crohn’s S-CM
Proliferation B A
IL-2
44.9
5.6
14.3
IL-2 + CD3
65.5
44.7
53.9
IL-2 + PHA
66.9
25.5
37.6
CD3 +
Media
(D. 3-7)
CD3 +
Normal S-CM
(D. 3-7) C
Normal S-CM
Crohn’s S-CM
IFN-g
p =0.002
p =0.007
p =0.0009
IL-2
CD3
PHA
% Inhibition of IFN-g Release
CD3 +
Crohn’s S-CM
(D. 3-7)
Days 0-3: IL-2 + CD3/CD28 or PHA
Days 3-7: CFSE + Crohn’s or Normal S-CM + CD3
Day 7: Harvest cells and measure proliferation and cytokines in supernatants

5. Supplemental Figure 4 C A B D 61.7 19.9 33.3 rhTGF-b (pg/mL) 57.6 56.450
500 C
50.4
56.5
56.4
57.6 A
61.7
19.9
Normal
S-CM
Normal S-CM
+ a-TGF-b
(200 mg/mL)
33.3 B D
a-TGF-b (mg/mL)
S-CM (250 mg/mL) + - 2 20
200
6000
3000
2000
1000
IFN-g (pg/mL)
rhTGF-b (pg/mL)
IFN-g (pg/mL)
media 5 50
500

6. Supplemental Figure 5 A B C D E F a-TGF-b (100 mg/mL) Purified IgG
Media
IFN-g (pg/mL)
a-TGF-b
(100 mg/mL)
Purified IgG
(100 mg/mL)
% Cells Proliferating
a-TGF-b
(100 mg/mL)
IgG
Media 61
64.1
65.9
Media D 61 E F
a-IL-6
(1 mg/mL)
a-IL-1
+ a-IL-1
Media
% Cells Proliferating
p = 0.06
a-IL-6
(1 mg/mL)
a-IL-1
+ a-IL-1
Media
IFN-g (pg/mL)
a-IL-6
(1 mg/mL)
a-IL-1
(1 mg/mL)
a-IL-6 + a-IL-1
(1 mg/mL)
66.8
70.4
71.8