2. Estimate % of your plate
Estimate % of your plate. Convert your % by using the multiplication values below.
For Example: Your 100mm plate is %90 full. If cells are collected in 10ml medium, 1 ml will cover %9 in another 100mm plate. If your transfer plate is 35mm plate instead of 100mm, then you have to multiply with the number below Your Plate corresponding to Transfer Plate. Number for 100mm to 355 mm is 6.9.
To convert 9% in 100mm to % in 35mm = Multiply %9 with 6.9 to get % in 35mm; which is 62.1.
Growth Area(cm2)
Transfer Plate
Your Plate
150mm plate
100mm plate
60mm plate
35 mm plate
Multiply with
3.8
12-well plate
To get
38.9
14.5
5.5
2.1
9.5
6-well plate
15.6
5.8
2.2
0.8 8
18.5
6.9
2.6
1.0 21
60 mm plate
7.0
0.4 55
100 mm plate
2.7
0.15
148
150 mm plate
0.14
0.05