1. The effect of mark enhancement techniques on the subsequent detection of semen/spermatozoa 
Rory Simmons, Paul Deacon, Darren J. Phillips, Kevin Farrugia 
Forensic Science International 
Volume 244, Pages (November 2014)
DOI: /j.forsciint
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2. Fig. 1 Layout used for creating a depletion series on substrates.
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3. Fig. 2
Semen marks 6–10 in the depletion series on grey laminate flooring aged 14 days and viewed under white light (top) and violet/blue light with yellow filter (bottom) post treatment: (a) before enhancement; (b) AV17; (c) AY7; (d) Lumicyano 4%; (e) cyanoacrylate/BY40; (f) black magnetic powder; and (g) iron-oxide powder suspension. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Forensic Science International  , DOI: ( /j.forsciint )
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4. Fig. 3
Semen marks 6–10 in the depletion series on white ceramic tile aged 14 days and viewed under white light (top) and violet/blue light with yellow filter (bottom) post treatment: (a) before enhancement; (b) AV17; (c) AV7; (d) Lumicyano 4%; (e) cyanoacrylate/BY40; (f) black magnetic powder; and (g) iron-oxide powder suspension. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Forensic Science International  , DOI: ( /j.forsciint )
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5. Fig. 4
Average number of semen marks observed before and after enhancement under white light across both substrates and all ageing periods.
Forensic Science International  , DOI: ( /j.forsciint )
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6. Fig. 5
Average number of semen marks observed before and after enhancement under violet/blue light (yellow filter) across both substrates and all ageing periods. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Forensic Science International  , DOI: ( /j.forsciint )
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7. Fig. 6
Semen marks 6–10 in the depletion series on white ceramic tile (top) and grey laminate flooring (bottom) aged 28 days and viewed: (a) under white light before Lumicyano fuming; (b) under violet/blue light (yellow filter) before Lumicyano fuming; (c) under white light post Lumicyano fuming; (d) under violet/blue light (yellow filter) post Lumicyano fuming; and (e) under blue/green light (orange filter) post Lumicyano fuming. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Forensic Science International  , DOI: ( /j.forsciint )
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8. Fig. 7
Semen marks 6–10 in the depletion series on white ceramic tile (top) and grey laminate flooring (bottom) aged 28 days and viewed: (a) under white light post cyanoacrylate fuming; (b) under violet/blue light (yellow filter) post cyanoacrylate fuming; (c) under white light post cyanoacrylate fuming/BY40; and (d) under violet/blue light (yellow filter) post cyanoacrylate fuming/BY40. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)
Forensic Science International  , DOI: ( /j.forsciint )
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9. Fig. 8
Average times recorded for a positive AP reaction for semen mark 1 in the depletion series across the various enhancement techniques, substrates and ageing periods.
Forensic Science International  , DOI: ( /j.forsciint )
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10. Fig. 9
Average times recorded for a positive AP reaction for semen mark 6 in the depletion series across the various enhancement techniques, substrates and ageing periods.
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11. Fig. 10
Average times recorded for a positive AP reaction for semen mark 10 in the depletion series across the various enhancement techniques, substrates and ageing periods.
Forensic Science International  , DOI: ( /j.forsciint )
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12. Fig. 11
Average grading for the detection of spermatozoa for semen mark 1 in the depletion series across the various enhancement techniques, substrates and ageing periods.
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13. Fig. 12
Average grading for the detection of spermatozoa for semen mark 6 in the depletion series across the various enhancement techniques, substrates and ageing periods.
Forensic Science International  , DOI: ( /j.forsciint )
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14. Fig. 13
Average grading for the detection of spermatozoa for semen mark 10 in the depletion series across the various enhancement techniques, substrates and ageing periods.
Forensic Science International  , DOI: ( /j.forsciint )
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15. Fig. 14
Microscopic examination of spermatozoa aged 14 days on grey laminate flooring, mark 1 in the depletion series following AV17 treatment and H&E staining. Recorded with Olympus BX51, 200× magnification.
Forensic Science International  , DOI: ( /j.forsciint )
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16. Fig. 15
Spermatozoa heads and tails aged 7 days on white ceramic tile, mark 1 in the depletion series observed following AY7 treatment and H&E staining. Recorded with Olympus BX51, 500× magnification.
Forensic Science International  , DOI: ( /j.forsciint )
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17. Fig. 16
Spermatozoa heads aged 7 days on white ceramic tile, mark 6 in the depletion series observed following Lumicyano 4% treatment and H&E staining. Recorded with Olympus BX51, 200× magnification.
Forensic Science International  , DOI: ( /j.forsciint )
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