1. Diagnosis of Esophageal Adenocarcinoma by Serum Proteomic Pattern
Zane T. Hammoud, MD, Lacey Dobrolecki, BS, Kenneth A. Kesler, MD, Emad Rahmani, MD, Karen Rieger, MD, Linda H. Malkas, PhD, Robert J. Hickey, PhD 
The Annals of Thoracic Surgery 
Volume 84, Issue 2, Pages (August 2007)
DOI: /j.athoracsur
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

2. Fig 1
Raw spectral data identifies 3 peaks using the H50 ProteinChip (Bio-Rad Laboratories, Hercules, CA). The top 2 specimens in each row are cancer, and the bottom 2 are normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

3. Fig 2
Cluster plots generated using H50 ProteinChip (Bio-Rad Laboratories, Hercules, CA) for the 3 peaks of interest.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

4. Fig 3
Decision tree analysis for H50 Protein Chip (Bio-Rad Laboratories, Hercules, CA) using the 3317, 4393, and 8632 peaks to identify cancer and normal specimens. The analysis correctly identified 42 of 43 cancers and 10 of 11 normals. The number after the peaks (eg, for 8632) represents the intensity of the peak. Class 0 = cancer; Class 1 = normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

5. Fig 4
Raw spectral data generated with IMAC30 ProteinChip (Bio-Rad Laboratories, Hercules, CA), showing 4 peaks (2743, 2861, 3217, 3421) that distinguish cancer from normal. The top 2 spectra in each graph are cancer, and the bottom 2 are normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

6. Fig 5
Cluster plots of the IMAC30 ProteinChip (Bio-Rad Laboratories, Hercules, CA) at the 4 peaks identified.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

7. Fig 6
Decision tree analysis for IMAC 30 Protein Chip (Bio-Rad Laboratories, Hercules, CA) at peaks 3217 and The analysis correctly identified 50 of 50 cancers and 10 of 10 normals. Class 0 = cancer; Class 1 = normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

8. Fig 7
Decision tree analysis using the IMAC30 ProteinChip (Bio-Rad Laboratories, Hercules, CA) and the peaks at 3217 and This analysis correctly identified 50 of 50 cancers and 9 of 10 normals. Class 0 = cancer; Class 1 = normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

9. Fig 8
Decision tree analysis using the IMAC30 ProteinChip (Bio-Rad Laboratories, Hercules, CA) and the peak at This analysis correctly identified 47 of 50 cancers and 9 of 10 normals. Class 0 = cancer; Class 1 = normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions

10. Fig 9
Decision tree analysis using IMAC 30 Protein Chip (Bio-Rad Laboratories, Hercules, CA) for 12 early stage tumors (T1 N0 and T2 N0) at peaks 2861 and This analysis correctly identified 11 of 12 cancers and 10 of 10 normals. Class 0 = cancer; Class 1 = normal.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2007 The Society of Thoracic Surgeons Terms and Conditions