1. Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
Altogen Biosystems offers the PC-12 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
PC-12 Transfection Reagent is a two component formulation that enhances lipid mediated transfection efficiency, and it is developed to provide high transfection efficiency with PC-12.
This cell line is a good host for studying adrenal glands and related adrenal gland cancers in particular.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase PC-12 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density PC-12 Cell Line
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
PC-12 Cell Line Description and Applications
The PC-12 cell line originates from a transplantable rat adrenal pheochromocytoma, and was established in The PC-12 cell line has a population doubling time is more than two days and will stop dividing and differentiate with a nerve growth factor treatment. In regards to differentiation, PC-12 cells will grow long neuritis and display cellular composition changes. The cells are small, have a rounded morphology, usually form in clusters and have limited cytoplasm. The PC-12 cell line is difficult to transfect, particularly after differentiation. This cell line has applications for studying adrenal glands and cancers related to adrenal glands.
High Density PC-12 Cell Line
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. PC-12 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
PC-12 Transfection Protocol
Plate 10, ,000 PC-12 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
Wash with 1xPBS and add 0.5 ml of fresh growth medium
Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium
Incubate transfection complexes at RT for minutes
Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
Add prepared transfection complexes to 0.5 ml of complete growth medium with PC-12 cells (from step 2)
Incubate cells at 37ºC in a humidified CO2 incubator
Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of PC-12 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. PC-12 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
PC-12 Transfection Kit Product Details
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
Data
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into PC-12 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
Data
Figure 2. Protein expression of Lamin A in PC-12 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into PC-12 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. PC-12 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > PC-12 Transfection Reagent (Rat Pheochromocytoma Cells)
PC-12 Transfection Kit Benefits
Pre-optimized transfection protocol for PC-12 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use PC-12 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable PC-12 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   