1. Cell Counts by Hemocytometer

2. 9 large squares – 1mm x 1mm
Center square divided into 25 smaller squares - 0.2mm x 0.2mm = 0.04 mm2
Depth of the counting chamber – 0.1 mm
Volume of a small square – 0.04 mm2 x 0.1 mm = mm3
1 ml = 1 cm3 = 1000 mm3 (10 mm = 1 cm)

3. 40x magnification
1 mm

4. 100x magnification
0.2 mm

5. 400x magnification

6. Zoox Density Calculations
Average of 10 counts of mm3 = 6 zoox
0.004 mm3 x 1 ml/1000mm3 = 4 x 10-6 ml
6 zoox/4 x 10-6 ml = 1.5 x 106 zoox/ml
Total homogenate volume = 5 ml
Total zoox = 5ml x 1.5 x 106 zoox/ml = 7.5 x 106 zoox
Wet weight of polyps = 0.25 g
Zoox density:
7.5 x 106 zoox/0.25 g wet wt. = 3.0 x 107 zoox/g wet wt.