1. CBTTC Expansion in China through BGI/CNBC
Charles Bao, Ph.D
General Manager
BGI Americas Corporation
2017 CBTTC Annual Investigator Meeting
May 8th, 2017

2. BGI Introduction
Established in 1999 with participation in the Human Genome Project
World’s largest genomic organization, experienced in genomic research, commercial NGS services, diagnostics, and sequencer manufacturing

3. BGI Quick Facts

4. China National Gene Bank (CNGB Overview)
Non-profit organization
Public Private Partnership (PPP)
Jointed funded by State Government, Shenzhen Municipal government and BGI
Operated by BGI Research
Missions:
To act as a platform for integrating genetic resources and capabilities
To lead the innovation, exploration and testing of new genomics technologies
To support the development of the life sciences and bio-economy in China
To maintain international competitiveness and influence in global genomics community

5. Platform and Infrastructure
Sequencing Platform
(reading)
Synthesis and Editing Platform
(writing)
Biorepository
(sample repository)
Database
(data repository)
Living biobank (living species repository)
Global collaborations
Collaborations over the platforms

6. CNGB - Biorepository
Automatic sample storage system with more than 10 million samples
Animal and plant genetic resources
386,000
Microbial resources
36,000
Plant germplasm resources (millet etc.)
5,000
Chinese unique genetic resources and
sample database of millet
Collected 2,540 millet samples
Sequenced 2,489 samples with 380Gb data
Opened samples and data to cooperative breeding institutions
The world’s largest repository of human enteric bacteria
Selected 17,000 bacterial strains
Sequenced 2,300 strain samples with 11.5 Gb data
Discovered 100+ new species
Genomics:
Deciphering the gut flora
Was named by Nature as “One of the top tem advancements in science and technology in the world”

7. CNGB - Sequencing Platform
BGISEQ-500
The BGI Desktop Sequencer with accuracy, flexibility, speed, simplicity and expandability
Sequencing capacity
150 BGISEQ-500 sequencers, with data output of ~5 PB per year

8. BGISEQ-500 Sequencing Applications
BGISEQ-500 PE100 Demo Data
Transcriptome:
Sample: UHRR（Universal Human Reference RNA）
Whole Exome:
Sample: (Genome in a Bottle) Human DNA- NA12878
Whole Genome:

9. CNGB - Data Repository and Analysis
Bio-informatics Data Center
46 open database
118 million page views
Highly efficient network
Cloud storage
Cloud computing
Aim to establish world’s first multi-omics cloud platform

10. BGI-Online Cloud Solution

11. BGI Online Demo Case for Speed Analysis
Benchmark: 1000 WES with 100X Analysis completed within 24 hours
Start
End
21h 47min 12sec
Autism Spectrum Disorder Project: 100 WGS with 30X Analysis completed within 17 hours

12. Overview of Beijing Tiantan Hospital
Founded in 1956, affiliated with Capital Medical University
A large first-class general hospital with neurosurgery specialty
Patient visits steadily increased tallying up to over 1,000,000 outpatient visits and 30,000 inpatient admissions per year
Founded by Dr. Zhongcheng Wang, Neurosurgery Center of Tiantan
Hospital has been recognized as the largest neurosurgical base in the world

13. Neurosurgical Center Pediatrics Brain trauma Spinal cord & column
Supratentorial tumours
Cerebrovascular disease
Pituitary adenoma and subtentorial tumors
Skull base and brain stem tumours
Endoscopy and functional disease
Intracranial diseases associated with ENT
Glioma center
Ward for officials and VIP

14. Neurosurgical Center
Over 12,000 operations in 2015, tumor 7569; Vascular diseases 1472; Functional neurosurgery 940; Brain trauma 1,025; Others 834
420 Beds
15 Subdivisons
170 Neurosurgeons:
55 full-time senior professors
50 associate professors
65 attending and residences

15. Pediatric Neurosurgery

16. CNGB – CBTTC collboration
Sample collection and biobanking
Data generation and analysis
Data integration via BGI-Online
Hospital enrollment and coordination
Joint Education
More Chinese hospital partners

17. Collaboration Outcome
CBTTC global network expansion into China
CBTTC diagnosis and treatment standard available to chinese pediatric brain tumor patients
Increased volume of data and clinical case from china expedites genetic discovery at CBTTC
Enrich CNGB biorepository and database resource