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Quantifying Mesenchymal Stem Cells in the Mononuclear Cell Fraction of Bone Marrow Samples Obtained for Cell Therapy  M. Alvarez-Viejo, Y. Menendez-Menendez,

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Presentation on theme: "Quantifying Mesenchymal Stem Cells in the Mononuclear Cell Fraction of Bone Marrow Samples Obtained for Cell Therapy  M. Alvarez-Viejo, Y. Menendez-Menendez,"— Presentation transcript:

1 Quantifying Mesenchymal Stem Cells in the Mononuclear Cell Fraction of Bone Marrow Samples Obtained for Cell Therapy  M. Alvarez-Viejo, Y. Menendez-Menendez, M.A. Blanco-Gelaz, A. Ferrero-Gutierrez, M.A. Fernandez-Rodriguez, J. Gala, J. Otero- Hernandez  Transplantation Proceedings  Volume 45, Issue 1, Pages (January 2013) DOI: /j.transproceed Copyright © 2013 Elsevier Inc. Terms and Conditions

2 Fig 1 Flow cytometry analysis conducted on BMMNC samples from: (A) Patient 9, in whom the highest percentage of CD271+ CD45− cells was observed, and (B) Patient 3, who showed the lowest percentage of CD271+ CD45− cells. Transplantation Proceedings  , DOI: ( /j.transproceed ) Copyright © 2013 Elsevier Inc. Terms and Conditions

3 Fig 2 Mean ± standard error of the number of mesenchymal stem cells detected in each patient according to the condition treated. Transplantation Proceedings  , DOI: ( /j.transproceed ) Copyright © 2013 Elsevier Inc. Terms and Conditions

4 Fig 3 Flow cytometry analysis of surface markers. Cells expressed CD73, CD90, and CD105 and lacked the expression of the hematopoietic markers CD34 and CD45. Panel (A) cytometry images corresponds to the isotype control, and panel B to the patient sample tested. Transplantation Proceedings  , DOI: ( /j.transproceed ) Copyright © 2013 Elsevier Inc. Terms and Conditions

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