1. Cagri Ozge Topal OSU ECEN 5060 Nanotechnology
NANOBIOSENSORS
Cagri Ozge Topal
OSU ECEN 5060 Nanotechnology

2. Abstract
The revolution of nanotechnology in molecular biology gives an
opportunity to detect and manipulate atoms and molecules at the
molecular and cellular level.
Definition of Nanobiosensor
History
Types of Nanobiosensors
Working Principle and Fabrication of Optical and FET Nanobiosensors
Application Areas and Current Research
Future Research

3. What is a Nanobiosensor?
A biosensor is a measurement system for the detection of an analyte that combines a biological component with a physicochemical detector, and a nanobiosensor is a biosensor that on the nano-scale size
Nanobiosensor
Transducer Detector Biological Recognition Element
(Bioreceptor)
Living biological system
(cell, tissue or whole organism)
Biological molecular species
(antibody, enzyme, protein…)

4. Biosensor Development
1916 First report on the immobilization of proteins: adsorption of invertase on activated charcoal.
1956 Invention of the first oxygen electrode [Leland Clark]
1962 First description of a biosensor: an amperometric enzyme electrode for glucose. [Leland Clark, New York Academy of Sciences Symposium]
1969 First potentiometric biosensor: urease immobilized on an ammonia electrode to detect urea. [Guilbault and Montalvo]
1970 Invention of the Ion-Selective Field-Effect Transistor (ISFET).
1972/5 First commercial biosensor: Yellow Springs Instruments glucose biosensor.
1976 First bedside artificial pancreas [Clemens et al.]
1980 First fiber optic pH sensor for in vivo blood gases.
1982 First fiber optic-based biosensor for glucose
1983 First surface plasmon resonance (SPR) immunosensor.
1987 Launch of the blood glucose biosensor[ MediSense]

5. Theory Principle of Detection Piezoelectric Mass
Electrochemical Electric distribution
Optical Light intensity
Calorimetric Heat
Types of Nanobiosensors
Optical Biosensors Nanotube Based Biosensors
Electrical Biosensors Viral Nanosensors
Electrochemical Biosensors Nanoshell Biosensors
Nanowire Biosensors

6. Theory
Optical Nanobiosensors
A sensor that uses light to detect the effect of a chemical on a biological system. [Kopelman et al.]
The small size of the optical fibers allow sensing intracelular intercelular physiological and biological parameter in micro- environment.
Two kind of fabrication methods for optical fiber tips;
1) Heat and Pull Method
2) Chemical Etching

7. Theory Nanowire Field Effect Nanobiosensors(FET) Sensing Element
Semiconductor channel (nanowire) of the transistor.
The semiconductor channel is fabricated using nanomaterials such
a carbon nanotubes,metal oxide nanowires or Si nanowires.
Very high surface to volume radio and very large portion of the atoms are located on the surface. Extremely sensitive to environment

8. Applications of Nanobiosensors
Biological Applications
DNA Sensors; Genetic monitoring, disease
Immunosensors; HIV, Hepatitis,other viral diseas, drug testing, environmental monitoring…
Cell-based Sensors; functional sensors, drug testing…
Point-of-care sensors; blood, urine, electrolytes, gases, steroids,
drugs, hormones, proteins, other…
Bacteria Sensors; (E-coli, streptococcus, other): food industry,
medicine, environmental, other.
Enzyme sensors; diabetics, drug testing, other.
Environmental Applications
Detection of environmental pollution and toxicity
Agricultural monitoring
Ground water screening
Ocean monitoring

9. Future Application Cancer Monitoring
Nanobiosensors play a very important role for early cancer detection in
body fluids.
The sensor is coated with a cancer-specific antibody or other
biorecognation ligands. The capture of a cancer cell or a target protein
yields electrical, optical or mechanical signal for detection. [Professor
Calum McNeil detection of cancer proteins that cause MRSA]
Identification of Biomarkers ↓
Validation of Cancer Biomarkers
Cancer Biomarkers
Ligands / Probes Developments
Cancer Diagnostics Biosensor ← Detector
Point of Care Cancer Diagnostics

10. References
Cullum, B.(2000). The development of optical nanosensors for biological measurements. Trends in Biotechnology, Vol 18,
Vo-Dihn, T.(2002).Nanobiosensors: Probing the sanctuary of individual living cells. Journal of Cellular Biochemistry Supplement, Vol. 39,
Pathak, P. et al.(2007). Cancer Research - Nanoparticles, nanobiosensors and their use in cancer research. Journal of Nanotechnology Online, Vol.3, 1-14.
Rogers, K.(2006). Recent advances in biosensor techniques for environmental monitoring. Analitica Chimica Acta. Vol. 568(1-2),
Li, C. (2005).Complementary Detection of Prostate-Specific Antigen Using In2O3 Nanowires and Carbon Nanotubes. Journal of the American Chemical Society, Vol.127(36),