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CELL STRUCTURE AND FUNCTION
CHAPTER THREE CELL STRUCTURE AND FUNCTION
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CONTRIBUTORS TO THE DISCOVERY OF CELLS
HOOKE _____________ LEEUWENHOEK _____________ SCHLEIDEN _____________ SCHWANN _____________ VIRCHOW _____________ RESPONSIBLE FOR CELL THEORY CORK ANIMALCULES Made better ______________ and observed cells in greater ______________. First to observe ______________ The first to __________ cells. Responsible for ____________ them Concluded that all ___________ ___________ were made up of ___________ Proposed that all cells come from ____________ __________ The first to note that _____________ were made up of ___________
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CELL THEORY ___________________________________________________________ 2. ___________________________________________________________ 3. ___________________________________________________________
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TOOLS OF BIOLOGY Microscope Function Magnifies up to… COMPOUND LIGHT
______________ microscope Uses light. __________ ______________ microscope Light cannot pass. _________ ______________ microscope Uses electrons __________ COMPOUND LIGHT 1000X STEREO 40X Also known as ______________ scope DISSECTING ELECTRON 500,000X COMPOUND LIGHT STEREOSCOPE ELECTRON MICROSCOPE
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LIGHT MICROSCOPE eyepiece Body Tube Turrett Arm Focus Objective
Low Objective Stage High Power Objective Stage Clips Course Adjustment Diaphragm Fine Adjustment Light Source Base
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(Independent Practice)
Labeling the Parts of the Microscope (Independent Practice) 1. EYEPIECE 2. BODYTUBE 3. TURRETT 10. ARM 11. FOCUS OBJECTIVE 4. LOW POWER OBJECTIVE 5. STAGE CLIPS HIGH POWER OBJECTIVE 12. 6. STAGE 13. COURSE ADJUSTMENT 7. DIAPHRAGM 8. LIGHT SOURCE 14. FINE ADJUSTMENT 9. BASE
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TOTAL MAGNIFICATION The focus objective focuses __________
The low power objective focuses _______ The high power objective focuses _______ 4X 10X 40X Keep in mind, there is also a lens in the EYEPIECE that focuses __________ “ON TOP OF” the magnification of the objective lenses. Therefore, _____________________________would be: _______________ X _________________ 10X TOTAL MAGNIFICATION EYEPIECE OBJECTIVE Practice: EYEPIECE X OBJECTIVE = TOTAL MAGNIFICATION TOTAL MAGNIFICATION OF FOCUS POWER __________ X __________ = ______________ TOTAL MAGNIFICATION OF LOW POWER TOTAL MAGNIFICATION OF HIGH POWER 10 4 40 X 10 X 10 X 100 X 10 X 40 X 400 X
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EYEPIECE: BODY TUBE: Where you place your eye. Contains ______ ______ that usually magnifies ______. Tube that supports the ______ _______ and connects it to the _________________. EYE PIECE ONE LENS TURRETT/NOSE PIECE 10x
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OBJECTIVES: STAGE CLIPS:
______________ that magnify objects to varying __________. FOCUS OBJECTIVE: ______________________________________________ LOW POWER OBJECTIVE: HIGH POWER OBJECTIVE: LENSES Holds the _____________ in place SLIDE “POWERS” SHORTEST LENS (4X) ONLY USED FOR SCANNING SMALL LENS (10 X) LOW MAGNIFYING POWER LONGEST LENS (40 X) HIGH MAGNIFYING POWER
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MAKES LARGE ADJUSTMENTS MAKES SMALL ADJUSTMENTS
BASE Knobs that make adjustments to the ______________ COURSE ADJUSTMENT ___________________________________________________________________________ FINE ADJUSTMENT FOCUS Supports the _____________ MICROSCOPE MAKES LARGE ADJUSTMENTS USED WITH FOCUS AND LOW POWER OBJECTIVES MAKES SMALL ADJUSTMENTS USED WITH HIGH POWER OBJECTIVE ONLY
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LIGHT SOURCE: Directs light up through the ______________ and through the ______________ so that it may be ______________ DIAPHRAGM SPECIMEN VIEWED
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Supports the __________________
STAGE: Supports the __________________ SLIDE/SPECIMEN
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NOSEPIECE: Also known as the _______________. It is the rotating device that holds the _____________/ (_________). TURRETT OBJECTIVES LENSES
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DIAPHRAGM An adjustable ________________ under the stage, allowing different __________ of __________ onto the stage. OPENING AMOUNTS LIGHT
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______________________________________________________________
arm - this attaches the eyepiece and body tube to the base. base - this supports the microscope. body tube - the tube that supports the eyepiece. coarse focus adjustment - a knob that makes large adjustments to the focus. diaphragm - an adjustable opening under the stage, allowing different amounts of light onto the stage. eyepiece - where you place your eye. fine focus adjustment - a knob that makes small adjustments to the focus (it is often smaller than the coarse focus knob). high-power objective - a large lens with high magnifying power. inclination joint - an adjustable joint that lets the arm tilt at various angles. low-power objective - a small lens with low magnifying power. mirror (or light source) - this directs light upwards onto the slide. revolving nosepiece - the rotating device that holds the objectives (lenses). stage - the platform on which a slide is placed. stage clips - metal clips that hold a slide securely onto the stage. _____________________________________________________________ __________________________________________________________ ____________________________________________________ ____________________________________________________ ________________________________________________________ ____________________________________________________ ____________________________________________________ ____________________________________________________ ____________________________________________________ ____________________________________________________ __________________________________________________________ ____________________________________________________
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arm - this attaches the eyepiece and body tube to the base
arm - this attaches the eyepiece and body tube to the base. base - this supports the microscope. body tube - the tube that supports the eyepiece. coarse focus adjustment - a knob that makes large adjustments to the focus. diaphragm - an adjustable opening under the stage, allowing different amounts of light onto the stage. eyepiece - where you place your eye. fine focus adjustment - a knob that makes small adjustments to the focus (it is often smaller than the coarse focus knob). high-power objective - a large lens with high magnifying power. inclination joint - an adjustable joint that lets the arm tilt at various angles. low-power objective - a small lens with low magnifying power. mirror (or light source) - this directs light upwards onto the slide. revolving nosepiece - the rotating device that holds the objectives (lenses). stage - the platform on which a slide is placed. stage clips - metal clips that hold a slide securely onto the stage.
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Types of cells Prokaryotic cells
primitive cells – “before the nucleus” No membrane bound organelles No nucleus Found in Eubacteria and Archaeabacteria Cell wall, cell membrane, cytoplasm, DNA & ribosomes
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Eukaryotic “true nucleus” Have membrane – bound organelles
All other kingdoms but Archaeabacteria & Eubacteria Organelles such as: Mitochondria, endoplasmic reticulum, vacuoles, lysosomes, and chloroplasts.
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Comparing the Two
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Structures common to both Prokaryotic and Eukaryotic cells
Cell Membrane Ribosomes DNA Cytoplasm Cell Walls (not in Animal or some Protist cells though!) Organelle – small structures in cells that have a specific function
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ANIMAL CELL PLANT CELL
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CELL MEMBRANE __________________________________________________________________________________________________ CELL MEMBRANE _______________
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____________________________________________
CYTOPLASM ________________________________________________________________________________________ ____________________________________________ CYTOPLASM _______________
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NUCLEUS ___________________________________________________
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______________________________ ______________________________
NUCLEAR MEMBRANE _________________________________________ Nuclear Membrane in Green ______________________________ ______________________________
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CHROMOSOMES ____________________________________________________________________________________ Chromosomes in Red _______________
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NUCLEOLUS _____________________________________ _______________
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MITOCHONDRIA __________________________________________________________________________ ____________
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LYSOSOMES ________________________________________________________________________________________ ____________
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_______________________________________
GOLGI APPARATUS _______________________________________ ______________
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ENDOPLASMIC RETICULUM
________________________________________________________________________________________ ____________________________________________ _______________
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RIBOSOMES __________________________________________________________________________________________________________________________________________________________________ _______________
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____________________________________________
CENTRIOLES ____________________________________________
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VACUOLE _______________________________________ ANIMAL CELL PLANT CELL
_______________________________________________________________________________________ ________ VACUOLE
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CELL WALL _________________________________________________________
__________________________________________________________ ____________
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Cytoskeleton A network of protein filaments that help the cell to maintain its shape. Centrioles – microtubules in animal cells that are involved in moving things during cell division. Also involved in movement.
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______________________
CHLOROPLAST ____________________________________________________________________________________________________________________________________ ______________________ CHLOROPLASTS
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ANIMAL CELL DIAGRAM Label the organelles of this animal cell.
_________________
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2.__________________ 1.__________________ 12.__________________ 3.__________________ 11.__________________ 4.__________________ 10.__________________ 5.__________________ 9.__________________ 8.__________________ 7.__________________ 6.__________________
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Structure of Lipid Bilayer
Section 3 Cell Organelles and Features Chapter 3 Structure of Lipid Bilayer
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Plasma Membrane, continued
Section 3 Cell Organelles and Features Chapter 4 Plasma Membrane, continued Membrane Proteins Cell membranes often contain proteins embedded within the phospholipid bilayer.-called integral prot
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Diffusion Section 3 Passive Transport Chapter 5 Passive transport – Involves the movement of molecules across the cell membrane without an input of energy from the cell.es the movement of molecules across cell membrane without an input of rDiffusion is the movement of molecules from an area of higher concentration to an area of lower concentration, driven by the molecules’ kinetic energy until equilibrium is reached. gy by the cell. is the movement of molecules from an area of higher concentration to an area of lower concentration, driven by the molecules’ kinetic energy until equilibrium is reached.
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Diffusion Concentration Gradient - The difference in the concentration of molecules across a distance. Equilibrium - When the concentration of molecules is the same throughout the space the molecules occupy. Once equilibrium is reached, molecules move randomely.
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Diffusion Across Membranes
Diffusion, continued Section 3 Passive Transport Chapter 5 Diffusion Across Membranes Molecules can diffuse across a cell membrane by dissolving in the phospholipid bilayer or by passing through pores in the membrane. Depends on size and type of molecule and on the chemical nature of the membrane.
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Diffusion Section 3 Passive Transport Chapter 5
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Osmosis is the diffusion of water across a membrane.
Section 3 Passive Transport Chapter 5 Osmosis is the diffusion of water across a membrane. No energy is required (passive transport).
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Direction of Osmosis Osmosis, continued Chapter 5
Section 3 Passive Transport Chapter 5 Direction of Osmosis The net direction of osmosis is determined by the relative solute concentrations on the two sides of the membrane.
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Direction of Osmosis Osmosis, continued Chapter 5
Section 3 Passive Transport Chapter 5 Direction of Osmosis When the solute concentration outside the cell is higher than that in the cytosol, the solution outside is hypertonic to the cytosol, and water will diffuse out of the cell.
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Direction of Osmosis Osmosis, continued Chapter 5
Section 3Passive Transport Chapter 5 Direction of Osmosis When the solute concentrations outside and inside the cell are equal, the solution outside is isotonic, and there will be no net movement of water.
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Hypertonic, Hypotonic, Isotonic Solutions
Section 3 Passive Transport Chapter 5 Hypertonic, Hypotonic, Isotonic Solutions
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How Cells Deal With Osmosis
Osmosis, continued Section 3 Passive Transport Chapter 5 How Cells Deal With Osmosis To remain alive, cells must compensate for the water that enters the cell in hypotonic environments and leaves the cell in hypertonic environments. Contractile vacuoles are organelles that regulate water levels in paramecia. This is active transport.
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Facilitated Diffusion
Section 3 Passive Transport Chapter 5 Facilitated Diffusion
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Chapter 5 Cell Membrane Pumps
Section 3 Active Transport Chapter 5 Cell Membrane Pumps Active transport moves molecules across the cell membrane from an area of lower concentration to an area of higher concentration. Unlike passive transport, active transport requires cells to expend energy.
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Cell Membrane Pumps, continued
Section 3 Active Transport Chapter 5 Cell Membrane Pumps, continued Some types of active transport are performed by carrier proteins called cell membrane pumps. Binds only to specific type of molecule. Usually the energy needed is provided by ATP.
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Cell Membrane Pumps, continued
Section 3 Active Transport Chapter 5 Cell Membrane Pumps, continued Sodium-Potassium Pump The sodium-potassium pump moves three Na+ ions into the cell’s external environment for every two K+ ions it moves into the cytosol. ATP supplies the energy that drives the pump. As a result, the outside of the membrane becomes positively charged and the inside negatively charged. This is important to transmit nerve impulses.
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Sodium-Potassium Pump
Section 3 Active Transport Chapter 5
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Chapter 5 Movement in Vesicles Endocytosis
Section 3 Active Transport Chapter 5 Movement in Vesicles Endocytosis In endocytosis, cells ingest external materials by folding around them and forming a pouch. The pouch then pinches off and becomes a membrane-bound organelle called a vesicle. Both endocytosis and exocytosis are used to transport large materials or large quantities of smaller materials.
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Movement in Vesicles, continued
Section 3 Active Transport Chapter 5 Movement in Vesicles, continued Endocytosis Endocytosis includes pinocytosis, in which the vesicle contains solutes or fluids, and phagocytosis, in which the vesicle contains large particles or cells. Some unicellular organisms feed by phagocytosis. Some animal cells called phagocytes, aid in destroying bacteria and viruses when they fuse with lysosomes.
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Movement in Vesicles, continued
Section 2 Active Transport Chapter 5 Movement in Vesicles, continued Exocytosis In exocytosis, vesicles made by the cell fuse with the cell membrane, releasing their contents into the external environment. Examples include exporting proteins outside the cell that were packaged in the golgi apparatus and exporting wastes and toxins from the cell.
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Endocytosis and Exocytosis
Section 2 Active Transport Chapter 5 Endocytosis and Exocytosis
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3.4 Diffusion and Osmosis KEY CONCEPT Materials move across membranes because of concentration differences.
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3.4 Diffusion and Osmosis Passive transport -
Molecules can move across the cell membrane through passive transport. There are two types of passive transport. 1) 2)
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3.4 Diffusion and Osmosis Osmosis – Diffusion –
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3.4 Diffusion and Osmosis There are three types of solutions.
isotonic – hypertonic – Hypotonic – Hypertonic Hypotonic
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3.4 Diffusion and Osmosis Some molecules can only diffuse through transport proteins. Some molecules cannot easily diffuse across the cell membrane. Facilitated diffusion -
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3.5 Active Transport, Endocytosis, and Exocytosis
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3.5 Active Transport, Endocytosis, and Exocytosis
A cell can import and export large materials or large amounts of material in vesicles during the processes of endocytosis and exocytosis. Cells use energy to transport material in vesicles. Exocytosis – Endocytosis – Phagocytosis -
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___________ - groups of cells that perform a similar function.
__________ - groups of tissues that perform a specific or related function. _______________ - groups of organs that carry out similar functions. vascular tissue leaf stem lateral roots primary root SYSTEMS root system shoot system CELL TISSUE ORGAN
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