1. Control of Microbial Growth
Chemical and
Chemotherapeutic Agents

2. Chemical Control MH (one per group) Culture Quadrants Swab Filter Disk
Pick one of 2
Coordinate with other table
Quadrants
Label for solutions
(A, B, C, D)
Label bacteria used
Swab
Confluent growth streak
Rotate 90°
Filter Disk
Dip in proper solution
Place in appropriate quad
Repeat for all 4 quads A C B D

3. Chemotherapeutic Agents
MH agar plate
Group: Pairs
Swab
Confluent
2x (90°)
AB discs
dispenser
seat discs

4. Chemical Agents MOA Isopropranol H2O2 Clorox Lab cleaner Other
Lysol
Betadine
Chlorhexidine
Denature, dissolve PM
Oxidative decarboxylation
Oxidation
Others
Disrupts plasma membranes

5. Minimum Inhibitory Concentration MIC

6. KB: measurements

7. Results Zones of inhibition Results Measure diameter Units: mm
Table of MIC
Interpretation
Susceptible
Intermediate
Resistant

8. Chemotherapeutic Agents MOA
Inhibit DNA gyrase
Inhibit Folic acid synthesis
30S binding/interferes with mRNA
Inhibit cell wall synthesis
PABA analog, blocks folic acid
30S binding/interferes with tRNA
50S binding/ prevents protein elongation
Naldixic acid
TMPS
Gentamycin
Vancomycin
Penicillin
Sulfisoxazole
Tetracycline
Azithromycin

9. Additive and Synergistic Effects of AB
Combination Therapy
Reduce resistance
Lower dosage
Enhance activity
Results
Zones of inhibition
Effects
Additive
Synergistic