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RECIPE Baupte Progress meeting 08-10/11/2004

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Presentation on theme: "RECIPE Baupte Progress meeting 08-10/11/2004"— Presentation transcript:

1 RECIPE Baupte Progress meeting 08-10/11/2004
UK progress Rebekka Artz Stephen Chapman Colin Campbell

2 I. WP01 UK Field site data Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland

3 Dark CO2 production 1st data point: 30/09/03

4 CO2 fluxes during photosynthesis

5 CO2 Net Ecosystem Exchange

6 CH4 Production exetainers exetainers exetainers exetainers

7 Water table 11/08/2003 13/11/2003 6/05/2004

8 Other parameters 1st data point: 18/12/03 1st data point: 12/02/04

9 Vegetation cover: Collars (0.07 m2)
B C D

10 Vegetation cover: Grids (1 m2)
B C D

11 II. WP2 Progress

12 Dark CO2 production

13 CO2 fluxes during photosynthesis

14 CH4 Production

15 Further work: 1st harvest expected to take place either end of november or mid march (pending decision) Clare Trinder will use the field site for litter bag experiments

16 III. Fungal community structure analysis
by DGGE of ribosomal 18S and/or ITS sequences

17 CVA of fungal ITS DGGE patterns per country, grouped by site.
Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).

18 III. Fungal community structure analysis
Clone library from DNA-derived ITS fragments has been prepared now cloned fragments are typed for RFLP type (in order to minimise no. of clones to be sequenced)

19 IV. Community level physiological profiling
Method optimised for use with peat; 48 h incubation period; 14C-labeled C sources used: N-Acetyl-Glucosamine Glucose Benzoic acid Galactose Phenylethylamine Arabinose Glycine Xylose Lysine Sucrose Arginine D-Mannitol Aspartic acid Glucosamine Glutamic acid

20 Other data are needed to perform CCA.

21 CVA of CLPP patterns per country, grouped by site.
Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).

22 V. FTIR (preliminary analysis)

23 CVA of FTIR patterns per country, grouped by site.
Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).


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