1. A B C D Cytoplasmic RTCs Cytoplasmic RTCs Nuclear RTCs
35000
30000
25000
20000
Early HIV-1 DNA (copies)
15000
10000
Mouse IgG1
Rabbit IgG1
5000
IP: MA CA RT IN
Vpr
PML
Ini1
cDNA recovery
(% of cRTC DNA)
4.66
2.25
2.79
6.03
5.52
4.23
6.59
0.61
0.95 B
Cytoplasmic RTCs
20000
16000
Late HIV-1 DNA (copies)
12000
8000
Mouse IgG1
Rabbit IgG1
4000
n.d.
IP: MA CA RT IN
Vpr
PML
Ini1
cDNA recovery
(% of cRTC DNA)
42.16
19.98
18.46
34.87
31.77
24.56
n.d
2.69
9.42
Cytoplasmic RTCs C
9000
8000
7000
6000
5000
Early HIV-1 DNA (copies)
4000
3000
2000
Mouse IgG1
Rabbit IgG1
1000
IP: MA CA RT IN
Vpr
PML
Ini1
cDNA recovery
(% of nRTC DNA)
85.14
2.0
7.31
35.25
40.54
52.52
77.58
8.74
13.04
Nuclear RTCs D
900
5 h post-infection (100%)
800
24 h p.i.
700
600
500
Early HIV-1 DNA (%)
400
300
200
Mouse IgG1
Rabbit IgG1
100
IP: RT IN
Vpr
cDNA recovery
(% of cRTC DNA)
1.11
0.12
3.14
22.84
1.46
3.38
0.55
1.41
Cytoplasmic RTCs