1. From: Retinal Localization and Copper-Dependent Relocalization of the Wilson and Menkes Disease Proteins
Invest. Ophthalmol. Vis. Sci ;47(7): doi: /iovs
Figure Legend:
RT-PCR amplification products of Menkes and Wilson genes separated by agarose gel electrophoresis. (A) RT-PCR products from mouse retinas and cultured mouse RPE cells. A band of the expected size (288 bp) is present in the amplification reaction using Menkes-specific primers and cDNA from both primary mouse RPE cells (mRPE) and mouse neurosensory retina without RPE (mRet). Primers specific for the Wilson gene generated a 180-bp amplification product with cDNA from mRPE but not mRet. A 50-bp increment ladder is shown for size reference. (B) RT-PCR products from ARPE-19 cells and human neurosensory retinas. Two sets of primers were used for the Menkes gene (M-1 and M-2) and two sets for the Wilson’s gene (W-1 and W-2). The cDNA template was from the source indicated at bottom. Bands of the expected size are present in the amplification reaction using Menkes-specific primers and cDNA from both ARPE-19 and human neurosensory retina without RPE (H-Ret). Primers specific for the Wilson gene generate an amplification product with cDNA from ARPE-19 but not H-Ret. A 100-bp increment ladder is shown for size reference.
Date of download: 11/7/2017
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