1. A. B.
BxPC-3
PANC-1
140
48 h
140
72 h
120
24 h
120
96 h
100
48 h
100
*** *
72 h
Cell viability (%) 80
***
***
Cell viability (%) 80
***
*** 60
***
*** 60 **
***
***
***
*** 40
***
***
*** 40
***
***
***
*** 20 20
***
***
***
***
***
***
***
***
0.2
0.4
0.6
0.8 1
0.25
0.5 1
2.5 5
AR-42 (μM)
AR-42 (μM)
Fig S1. Effects of AR-42 on cell viability of 2 pancreatic cancer cell lines. MTT assay analyzing the effect of AR-42 on (A) BxPC-3 and (B) PANC-1 cell viability. The IC50 of AR-42 for BxPC-3 were 0.74, 0.52, 0.29 μM at 24, 48, and 72 h, respectively. The IC50 of AR-42 for PANC-1 were 0.67 and 0.18 μM at 72 and 96 h, respectively. The inhibition of PANC-1 cell viability did not reach 50% at 48 h. Data are presented as mean ± S.D. from three individual experiments. * P < 0.05, ** P < 0.01, *** P < compared to the control group.