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Lesson 3: The Plant Cell... Teacher: Sven Beer סוון בר ,
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For this lesson read: Biology of Plants – 6th ed. pp 7th ed. pp 8th ed. pp Only text on: Plastids (chloroplast), vacuole, cell wall and plasmodesmata! For next lesson read: Biology of Plants – 6th ed. pp 7th ed. pp 8th ed. pp
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The Plant Cell Plasmodesmata
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The (Plant) Cell… The Prokaryotic “Plant” Cell
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Endosymbiosis Cyanobacteria 28.2
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1: Chloroplasts (plastid)
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DEMO !!! Chloroplast 1.mov
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Ulva chloroplasts
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Chloroplast: The Site of Photosynthesis
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The Structure of the Chloroplast
Stroma Granum / Grana = Thylakoid stack / stacks
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The Chlorophyll Structure
Hydrophilic Hydrophobic
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Chlorophyll MW ~ 950 Hydrophilic Hydrophobic
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CO2 + H2O >>> CH2O + O2
photo - synthesis Light Reactions Carbon Fixation and Reduction Reactions (Dark reactions) stroma grana CO2 Fixation
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Chloroplasts are one form of plastids
Cold Prolamellar bodies Etioplast Light
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Chloroplasts contain chlorophyll and carotenoids,
in cold: chloroplast can not form chlorophyll and carotenoids bereak down slowly; chromoplast are formed from chloroplasts
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Plasmodesmata
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2: Vacuoles Vacuoles a) contain waste products; b) keep turgor
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Waste products, antocyanins
salts
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Up to 90% of cell volume!
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Autumn Colors: Chlorophyll is not formed in the cold – carotenoids break down slowly >> chromoplasts (yellow / orange) Sugars (and other organic compounds) form antocyanins (red) in the vacuoles
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red orange
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Vascular bundle! Vascular bundle! Cell Growth = vacuole expansion / A land plant will wilt in seawater!
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3) Cell walls
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Secretory Vehicles contain: Cellulose, Hemicellulose, Pectin and Glucoproteins
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Primary wall Secondary wall Middle Lamella
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Macrofibirls>>Microfibrils>>Micelles>>Cellulose
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Cellulose (more in secondary walls)
cross-linked by Hemicellulose(s) = xyloglucans (glucose + xylose) Pectins (in primary cell walls only) = hydrophilic polysaccharides Glycoproteins (in primary walls) Lignins (make for stiffness, more in secondary walls) Cutins, Suberins (cork) and Waxes Primary wall (while the cell enlarges) Secondary wall (when the cell has reached its final size)
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Microfibrils, macrofibrils, lignin, glucoproteins
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Plasmodesmata
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Plasmodesmata Pit
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Marine Botany Lesson 3 (cont): The physical and chemical environment relevant to marine plant growth in the oceans…
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Abiotic = Physical / Chemical Conditions affecting Plant Growth
Light – Irradiance Temperature Salinity Pressure Currents pH?
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Light ? Photons Quanta
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Light, both electromagnetic waves and particles (quanta),
“that trigger nerve impulses from the retina” Quanta Photons
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Leaves are green! Eye
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Why are leaves green? Tomatoes (and Red Algae) red?
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Visible radiation (= “light”)
Quanta Photons Photosynthetically Active Radiation - PAR ( nm)
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Light, both electromagnetic waves and particles (quanta),
“that trigger nerve impulses from the retina” Photons Quanta λ Quantum E = h*c / λ Photon(s) = quanta ( nm)
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Chlorophylls absorb light at ~450 and ~670 nm… but how do we know that THIS light drives photosynthesis???
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Engelmann ~ (1890)
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Absorption and Action Spectra
Absorption Spectra Action Spectrum Engelman -1883 Filament of the alga Spirogyra
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Red algae and Cyanobacteria
Chlorophyll Chlorophyll Phycobilins
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Red algae: Complementary chromatic adaptation (?)
Phycobilins
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Measuring Light (Ft. Candles) (Energy)
Photon Flux (mol photons m-2 s-1); [mol photons = Einstein (E)] Sunny day: ~ 2000 mmol photons m-2 s-1 Demo
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Quantum Yield Absorption vs. Quantum Yield….
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