1. 《Plant tissue culture》Chapter 1 Introduction
——keynote speaker
Hong Sen-rong（Associate professor ）
（College of Life Sciences ）

2. Totipotency
Growth regulators
Plant tissue culture

3. Section 1 Basic concept of plant tissue culture
Sterile, manual control, culture medium, regenerated cells or whole plants.
Also known as plant culture in vitro.

5. 2、Characteristics of plant tissue culture
（1）Sterile
（2）Artificial culture medium
（3）Culture materials：Organ，tissue，cell
—— explants

6. （4）Clone or organ (root and bud)
（5）Enclosed container
（6）Temperature, light intensity, time: all been artificially set.

7. 3、Research types and tasks of plant tissue culture
②Organ culture
③Embryo culture
④Cell culture
⑤Protoplast culture

8. （2）Research task Production of secondary metabolites
Germplasm innovation and variety breeding
Rapid propagation
Virus-free plantlet cultivation
Artificial seed production
Conservation in vitro of Germplasm Resources

9. Section 2 Formation and development of plant tissue culture
1、Pioneering of plant tissue culture
（1）Cell theory
（2）First papers on plant tissue culture：

10. G. Haberlandt，1902，Germany
Put forward： Totipotency

11. Experiment material ：Wild sesame野芝麻

12. Experiment material ： dayflower鸭跖草

13. Experiment material ： Eichhornia凤眼兰

14. Experiment material： Ornithogalum caudatum
虎眼万年青

15. Selected materials：
Palisade cell

16. Epidermal cells

17. Epidermal hair

18. Knop culture medium（balanced nutrient solution）
1865，Knop， a variety of chemical composition （proportion of different components）
But it is a pity for him to fail?
WHY?

19. 2、foundation of plant tissue culture
1933: China, Li Jidong and Shen Tong: the use of natural extracts of tissue culture.

20. 1926-1948: Plant hormones have been found.
Auxin——IAA

21. 1953: W.H.Muir，liquid culture.
Cell suspension culture

22. 1958: The discovery of somatic embryo.

25. Similar to zygotic embryo

26. 3、The establishment of plant tissue culture
1960，G. Morel. He obtained the PLBs by shoot tip culture, cultured Cymbidium hubridum virus-free plants and achieved a rapid propagation method, which developed into the orchid industry.

27. PLBs
protocorm-like bodies
Cymbidium hubridum

29. 1962: Murashinge and Skoog, medium in tobacco, is now widely used
—— famous MS medium.

30. 1960：E. C. Cocking started protoplast culture.

31. 1972：P.S.Carlson, protoplast fusion.

32. ：Pollen culture.

33. 1969：The major medicinal components were produced in cell suspension culture.

34. Section 3 Application and prospect of plant tissue culture
1、 Rapid propagation in-vitro
2、Virus-free plantlet cultivation
3、Cultivation of new varieties and new species

35. Variety improvement and distant hybridization
Selection in vitro of mutants
Haploid 单倍体 breeding

36. 4、Secondary metabolite production
Ginsenoside人参皂苷 , paclitaxel紫杉醇 , shikonin 紫草素 etc..
5、conservation In vitro of plant germplasm resources
Save manpower, material resources and land.

37. 6、Artificial seed：1978 Murashinge.

41. New germplasms were created by plant gene engineering techniques;

42. Plant cell culture and its production of secondary metabolites on a large scale.

43. See you next week！