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MOST PROBABLE NUMBER (MPN) METHOD
statistical method usually higher counts than SPC masses of glassware no colony morphology replicate tubes inoculated with dilutions (3-5 replicates) growth in tube shown by some form of change in media eg turbidity / gas production Positive tubes refer to MPN tables to give MPN/g Can be used with any selective media for enumeration
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Flow chart of Presumptive MPN
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Flow chart of Confirmed and Completed MPN
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MPN tables refer to Australian Standard 1766. 1
MPN tables refer to Australian Standard Estimation of Most Probable Number of Microorganisms Tables 1 and 2
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