1. Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Altogen Biosystems offers the Fibroblast Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The Fibroblast Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfection efficiency with the Fibroblast cell line.
This cell line is a good host for studying fibroblast. When cultured in vitro, Fibroblast cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase Fibroblast Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. Fibroblast Cell Line Description and Applications
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Fibroblast Cell Line Description and Applications
Fibroblast cells contain one or two nucleoli and have a branched cytoplasm surrounding the nucleoli. The cells maintain connective tissue structure via the excretion of extracellular matrix components. Connective tissue and fibroblasts are the primary components of organisms. When active Fibroblasts have rough endoplasmic reticulum. Densely collected fibroblasts frequently organize in parallel clusters. Fibroblasts display varying phenotypes depending on anatomical location. Moreover, fibroblasts can contribute to basal lamina in some scenarios.
Fibroblast cells
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. Fibroblast Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Fibroblast Transfection Protocol
1. Plate 10, ,000 Fibroblast cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with Fibroblast cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of Fibroblast cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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5. Fibroblast Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Fibroblast Transfection Kit Product Details
Proprietary cationic lipids formulation
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Transfection kit includes Transfection Enhancer reagent
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Data Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Data
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into fibroblast cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).

7. Data Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Data
Figure 2. Protein expression of Lamin A/C in Fibroblast cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into Fibroblast cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. Fibroblast Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Fibroblast Transfection Reagent (Fibroblast Cells)
Fibroblast Transfection Kit Benefits
Pre-optimized transfection protocol for Fibroblast cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use Fibroblast transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable Fibroblast cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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