1. SunBio
Technology Presentation
SunBio
mPEG-20K
2003

2. mPEG 20K Specification Parameter Instrument Specification Result
Molecular Weight (Mw)
MALDI-TOF
20,000 +/- 2,000
21,085
Polydispersity
GPC
<1.1
1.08
1.01
Main Peak Fraction
>90%
100%
Diol Content
NMR
<2%
Undetectable 1%
Water Content
Karl Fisher Method
<1%
0.2%
Color
Visual
White
pass

3. mPEG 20K – Main Peak Fraction & PDI by GPC
Data analysis
Peak Information
Analysis Result
Main Peak Fraction: 100%
Diol Content: undetectable
Polydispersity: 1.08
Main Peak Fraction
Polydispersity

4. mPEG 20K – MW & PDI by MALDI-TOF Mass
21,085
Lot #: E K ( )
Mw: 21,085
Polydispersity: 1.01

5. mPEG 20K – Diol Content by NMR
(Step 3) -OH Peak
is now read:
(Step 1) PEG Backbone Peak
(MW is obtained from MALDI-TOF)
(Step 2) Methoxy Peak
is set at 3
In theory, with the methoxy peak (3H) set at 3, the –OH peak (1H) should be 1.0
When the –OH peak reads more than 1.0, it indicates presence of diol. If the –OH value is 1.10, the diol content is calculated as 5%.