1. Looking at the small stuff
Microscopy
Looking at the small stuff

2. Microscopes “micro” means : “scope” means:
Small
“scope” means:
Watch/ look
It was actually developed first as entertainment for the rich

3. Light Microscope Focuses visible light rays
Can be used on live or dead specimens
Dyes and fluorescent markers help for viewing
Magnify up to 1000X on the ones we have
Examples:
Compound light microscope
Confocal microscope

4. Light Microscope

5. Light Micrographs
Plant magnified
Human chromosomes 

6. Confocal Microscope

7. Confocal micrograph Spirogyra crassa
Maximum projection green: Actin. Rhodamin, Label: Phalloidin. Actin red: Chloroplasts. Autofluorescence Image acquired with the Leica TCS SP2

8. Electron Microscopy (EM)
Focus beam of electrons on the specimen
Specimens must be dead
Kinds
Transmission Electron Microscope (TEM)
Cross-section of the material
Most detailed of internal of cell
Scanning Electron Microscope (SEM)
Surface of the specimen
Shows texture, but less detail

9. TEM

10. TEM

11. SEM

12. SEM
Ant
Pollen Grains

13. Red Blood Cells
SEM
Compound Light
TEM

14. Compare cardiac myocytes
Confocal microscope
Compound light microscope
neonatal cardiac myocytes from rat
green: Myomesin,
red: Cadherin
blue: DNA (nuclei),

15. Keen eyes
Light microscopes- will not show you something the size of a virus! Do not display in great detail.
Confocal microscopy uses brightly colored markers
TEM is detailed, flat, and black and white
SEM is 3D, textured, and black and white