Presentation is loading. Please wait.

Presentation is loading. Please wait.

Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells  Anthony G. Passerini, PhDa,

Published byEverett Gallagher Modified over 6 years ago

Similar presentations

Presentation on theme: "Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells  Anthony G. Passerini, PhDa,"— Presentation transcript:

1 Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells  Anthony G. Passerini, PhDa, Amy Milsted, PhDb, Stanley E. Rittgers, PhDa  Journal of Vascular Surgery  Volume 37, Issue 1, Pages (January 2003) DOI: /mva Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

2 Fig. 1 Schematic shows shear stress protocol applied to HUVEC monolayers. On reaching confluency in static culture, cells were preconditioned at normalized arterial WSS of 15 dynes/cm2for 24 hours to reset baseline gene expression. They then were exposed to various step changes in WSS for 6 hours. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

3 Fig. 2 Mean ± standard deviation expression levels for endothelial genes (n = 3 per gene per shear level). A, END1; B, NOS3; C, PDGFA; D, PDGFB; E, FGF1; and F, FGF2. *P <.05 from baseline (15 dynes/cm2) in preconditioned cells. Expression levels in static cultured cells are shown for comparison only. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

4 Fig. 3 Mean ± standard deviation expression levels for endothelial genes (n = 3 per gene per shear level). A, TGFA; B, TGFB1; C, VEGF; D, IGF1; E, EGF; and F, ACE. *P <.05 from baseline (15 dynes/cm2) in preconditioned cells. Expression levels in static cultured cells are shown for comparison only. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

5 Fig. 4 Mean ± standard deviation END1/NOS3 mRNA expression ratio (n = 3 per shear level). *P <.05 from baseline (15 dynes/cm2) in preconditioned cells. END1/NOS3 expression ratio in static cultured cells is shown for comparison only. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Download ppt "Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells  Anthony G. Passerini, PhDa,"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages 1072-1082 (October 2014) DOI:

Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages (October 2014) DOI:
The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
I may not be Southern by birth, but I am proud to be an American: The role of international medical graduates in vascular surgery and the Southern Association.

I may not be Southern by birth, but I am proud to be an American: The role of international medical graduates in vascular surgery and the Southern Association.
S. Sulaiman Shoab, MD, FCPS, FRCS, J. H. Scurr, MS, FRCS, P. D

S. Sulaiman Shoab, MD, FCPS, FRCS, J. H. Scurr, MS, FRCS, P. D
Persistent plasminogen activator inhibitor 1 gene expression in cardiac transplant recipients with idiopathic dilated cardiomyopathy  Romana Schäfer,

Persistent plasminogen activator inhibitor 1 gene expression in cardiac transplant recipients with idiopathic dilated cardiomyopathy  Romana Schäfer,
Recurrent thromboembolism in patients with vena cava filters

Recurrent thromboembolism in patients with vena cava filters
Insulin action enhancement normalizes brachial artery vasoactivity in patients with peripheral vascular disease and occult diabetes  Ricardo Avena, MD,

Insulin action enhancement normalizes brachial artery vasoactivity in patients with peripheral vascular disease and occult diabetes  Ricardo Avena, MD,
Vessel loops made easy Journal of Vascular Surgery

Vessel loops made easy Journal of Vascular Surgery
A percutaneous arterial closure protocol can decrease complications after endovascular interventions in vascular surgery patients  Philip P. Goodney,

A percutaneous arterial closure protocol can decrease complications after endovascular interventions in vascular surgery patients  Philip P. Goodney,
Somatostatin gene transfer and expression in endothelial cells

Somatostatin gene transfer and expression in endothelial cells
Intestinal permeability is increased in patients with intermittent claudication  Hirohide Iwata, MD, Masahiro Matsushita, MD, Naomichi Nishikimi, MD, Tsunehisa.

Intestinal permeability is increased in patients with intermittent claudication  Hirohide Iwata, MD, Masahiro Matsushita, MD, Naomichi Nishikimi, MD, Tsunehisa.
Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M
Angiogenic response induced by mechanical transmyocardial revascularization  Victor Chu, MDa, Jin-qiang Kuang, MDa, Amy McGinna, Adel Giaid, PhDb, Stephen.

Angiogenic response induced by mechanical transmyocardial revascularization  Victor Chu, MDa, Jin-qiang Kuang, MDa, Amy McGinna, Adel Giaid, PhDb, Stephen.
The percutaneous Greenfield filter: Outcomes and practice patterns

The percutaneous Greenfield filter: Outcomes and practice patterns
Journal of Vascular Surgery

Journal of Vascular Surgery
Asymptomatic internal carotid artery aneurysm

Asymptomatic internal carotid artery aneurysm
Thrombolysis for deep venous thrombosis

Thrombolysis for deep venous thrombosis
The inflammatory response to upper and lower limb exercise and the effects of exercise training in patients with claudication  Shah Nawaz, FRCSa, Richard.

The inflammatory response to upper and lower limb exercise and the effects of exercise training in patients with claudication  Shah Nawaz, FRCSa, Richard.
An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function  Guohao Dai, MSa, Olga Tsukurov, MDb,

An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function  Guohao Dai, MSa, Olga Tsukurov, MDb,

Similar presentations

Ads by Google