1. MINX RNA nt
120 nt
74 nt
45 nt M M
250 nt
150 nt
239 nt MINX RNA
200 nt
100 nt
150 nt
50 nt
Background dot
100 nt
50 nt
6% PAGE 8M urea
13% PAGE 8M urea
Lanes M- 50 bp DNA ladder NEB, 1-MINX RNA control (untreated),
2-MINX+Cyto fraction (negative control), 3-HeLa NE Batch I, 4- Hela Ne Batch 2,
6 & 8 - HeLa NE Batch 1 & 2 respectively (duplicate reaction another day).
Has splicing worked here? If yes, what are the bands of interest? I don’t see
bands corresponding to lauriate intron and intermediate which form
bands above substrate as seen in many articles. Pls help
Reaction was at 30degree Celsius for 3 hours with 80 ug protein (Ne-50% reaction volume)