1. Clinical Practice Microbiology
Prof. Dalia Mohsen
Prof. in Microbiology
CLS 417

3. E. mail: Dr.dalia@inaya.edu.sa
Welcome to CLS 417
Prof. Dalia M. Mohsen
Prof. in Microbiology
E. mail:
Office:- 2B-111

4. OBJECTIVES
Students should know the specimens received in the bacteriology laboratory, their collection techniques, suitability, and processing.
Introduction to the safety measures implemented in the laboratories.
The culture media preparation and sterilization.
processing of specimens, isolation/ identification of organisms, and antibiotic susceptibility testing.
The diagnostic techniques in the routine Clinical Immunology, Mycology and Virology laboratories will also be taught.

5. What this?

7. OVERVIEW Microbiology:-
Microbiology can be defined as the biology of microscopic organisms, or life too small to be seen with the naked eye.
Microbiology covers several disciplines, including virology (study of viruses), bacteriology (study of bacteria), mycology (study of fungi), and parasitology (study of parasites).

8. Clinical microbiology:-
 Is a branch that concerned with the prevention, diagnosis and treatment of infectious diseases.
In addition, this field of science studies various clinical applications of microbes for the improvement of health.

9. Microbiology scientist
Antonie van Leeuwenhoek
The "Father of Microbiology, born in Delft, Dutch Republic
considered to be the first microbiologist.
He is best known for his work on the improvement of the microscope first to observe and describe single-celled organisms

10. Antonie van Leeuwenhoek

11. Louis Pasteur:-
"French chemist was the founder of microbiological sciences. discoveries of the principles of vaccination, microbial fermentation and pasteurization.
Robert Koch:-
 German physician The founder of modern bacteriology, he is known for his role in identifying the specific causative agents of tuberculosis, cholera, and anthrax and for giving experimental support for the concept of infectious disease.

12. Louis Pasteur

13. Robert Koch  

14. Marks’ Distribution First assessment 10 marks Second assessment
quizzes
projects
Final (practical) exam
20 marks
Final (theory) exam
40 marks
Total
100marks

15. Topics to be covered

16. General bench specimens
Bacteriological culture media
Urine specimen
Blood culture
Stool specimen
General bench specimens
Serological test
Review in diagnosis of viral infection
Review in diagnosis of fungal infection

17. Required Textbook
Cossart, P., P. Boquet, S. Normark, and R. Rappuolo. Cellular Microbiology. ASM Press, Washington, DC, 2000
Vandepitte ,J and Verhaegen J,Engbaek K,Rohner P,Poit P,and C C. Heuck. .Basic laboratory in Clinical Bacteriology.WHO,Geneva,2nd Edition 2003
Kaufmann, S. H. E., A. Sher and R. Ahmed. Immunology of Infectious Diseases. ASM Press, Washington, DC, 2001
Madigan, M. T., Martinko, J. M., and J. Parker. Brock Biology of Microorganisms. Prentice Hall, Upper Saddle River, NJ, 1997
Mims, C., A. Nash, and J. Stephen. Mims' Pathogenesis of Infectious Disease. Academic Press, San Diego, CA. 2001