1. Genomic and cDNA Libraries
Chapter 3
Genomic and cDNA Libraries
© 2012 John Wiley & Sons, Ltd.

2. Figure 3.1 Making a genomic library.

3. Figure 3.2 Genomic libraries: overlapping and non-overlapping fragments.

4. Figure 3.3 Using a partial digest to produce a collection of overlapping fragments.

5. Figure 3.4 Amplification of gene libraries.

6. Figure 3.5 Assessing the quality of a gene library.

7. Figure 3.6 Principle of oligo(dT) purification of mRNA.

8. Figure 3.7 Purification of mRNA through an oligo(dT) column.

9. Figure 3.8 Synthesis of cDNA from mRNA.

10. Figure 3.9 cDNA synthesis: using homopolymer tailing.

11. Figure 3.10 Cloning cDNA.

12. Figure 3. 11 cDNA synthesis: enhancing representation of 5 mRNA ends
Figure 3.11 cDNA synthesis: enhancing representation of 5 mRNA ends. RT, reverse transcriptase.

13. Figure 3.12 Melting (denaturation) of DNA.

14. Figure 3.13 Strength of association of base pairs.

15. Figure 3.14 Formation of hybrid DNA between similar but non-identical DNA molecules.

16. Figure 3.15 High and low stringency washing.

17. Figure 3.16 Screening a gene library by hybridization with a gene probe.

18. Figure 3.17 Southern blotting.