1. Actual Animal Genetic Resources in Slovakia
P. Chrenek, Makarevich A., Bulla J.
DAGENE 2016

2. Introduction
Ratification of the Convention on the Biodiversity was confirmed by Slovak Republic also in 1994, to protect biodiversity, to guarantee sustainable use of its components include also animal genetic resources.
Generally, the importance of animal genetic resources protection emerge from biological, economic, landscaping and cultural needs of each country that are realized using In situ and ex situ preservation including national gene banks (NGB).

3. Introduction
National Gene Bank should guarantee monitoring, collection and preservation of samples from genetic resources (spermatozoa, oocytes, embryos, somatic cells, tissues, DNA etc.) in original condition, deeply frozen or lyophilized; their storage, molecular-genetic characterization of samples, research-comparison of the genetic biodiversity, management of information system about stored samples and their use for original breed restoration.

4. Introduction
In the last fifty years there was a significant decrease in the animal number in Slovak Republic and in some species the origin, autochthonous breeds completely disappeared. The possible extinction of these breeds would also mean irrecoverable loss of the genetic variability and so the loss of unique gene and allele combinations that would be very useful in the future e.g. for the generation of new farm animal genotypes.
Wallachian sheep
Slovak pinzgauer

5. Actual Animal Genetic Resorces in Slovak Republic 2015
Specie/ Breed
Total number of animals
Number of Females in control of yield
cattle/ Pinzgauer
9883
1583
cattle/ Slovak Spoted
161631
30620
sheep/ Walachian sheep
2391
383
sheep/ Thoroughbred valaška
130207
7354
sheep/ Cigaja
122253
6320
goat/ White shorthaired
8389
843
goat/ Brown shorthaired
1485 70
horse/ Furioso
181
horse/ Hucul
107
horse/ Lipican
163
horse/ Nonius
80-110 29
horse/ Norik muransky
110
horse/ Slovak teplokrvník
895
horse/ Slovak sport pony 76
hen/ Oravka -
gous/ Slovak white
rabbit/ Nitra
rabbit/ Zobor
80-120
rabbit/ Zempliner
EFABIS (

6. Introduction
The Slovak Pinzgauer cattle, Wallachian sheep (Valaška), belong to these endangered breeds in Slovak republic.

7. Introduction
Oravka hen,Zobor or Nitra rabbit belong to these endangered breeds in Slovak republic

8. Animal Gene Bank in Nitra, Slovak Republic
(Chrenek P. et al., Zygote 2013)

9. Analysis of rabbit transgenic and non-transgenic mammary glandMi V M G N ER L P Nc
Fig. 4. Electron micrograph of transgenic (A) and non-transgenic (B) rabbit mammary gland tissues on D35 of third lactation.
Each micrograph shows a supranuclear area with a higher presence of mitochondria (M) in the transgenic tissues. Protein globules (P) are seen in the lumen (L) of the alveolus in the non-transgenic tissue. Nucleus (N), Golgi apparatus (G), rough endoplasmic reticulum (ER), and display synthetic activity. Vacuoles (V) and microvilli (Mi) are seen. Magnification: x
Chrenek et al., AHE, 2010

10. (Kulíkova B.,..,Chrenek P. et al., Theriogenology 2015)
Application of biotechnological method for cryopreservation of animal genetic resources
1) FAO database (
2) DNA analysis (microsatelities)
3) Cryopreservation (Vitrification) of animal embryos, sperm and stem cells
Nitra rabbit
Zobor rabbit
(Kulíkova B.,..,Chrenek P. et al., Theriogenology 2015)

11. Animal Gene Bank in Nitra, Slovak Republic

12. Thank you for your attention