1. Ultrathin sections of proximal convoluted tubule cells incubated with anti-gp330 antibody followed by protein A–gold, after embedding in Lowicryl K4M, from a control rat (A) and after maleate treatment for 45 min (B). Panel A shows that, in normal rats, gold particles, revealing antigenic sites, are localized on the brush border (arrowheads), as well as in apical invaginations and vesicles (arrows). Note the uniform distribution of labeling in brush border. All microvilli are labeled. Note presence of gold particles in apical vesicles and their absence from intercellular space (asterisk). Panel B shows a proximal tubule cell of a maleate-treated kidney (45 min). Gold particles are still present in most apical vacuoles (arrows) of this cell and implies that maleate does not inhibit the transfer of megalin and its ligands into the endosomes. (Magnifications: A: × 25,000; B: × 15,000.)
Source: The Renal Fanconi Syndrome, The Online Metabolic and Molecular Bases of Inherited Disease
Citation: Valle D, Beaudet AL, Vogelstein B, Kinzler KW, Antonarakis SE, Ballabio A, Gibson K, Mitchell G. The Online Metabolic and Molecular Bases of Inherited Disease; 2014 Available at: Accessed: November 09, 2017
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